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Literature DB >> 7236205

Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus.

I Mains, D M Power, E W Thomas, J A Buswell.

Abstract

An NADH-(dichlorophenol-indophenol) oxidoreductase was purified 104-fold and in 25% overall yield from the thermophilic bacterium Bacillus stearothermophilus, strain PH24. After solubilization in 2M-NaCl at 70 degrees C, the enzyme was purified by ion-exchange and hydroxyapatite chromatography, followed by affinity chromatography on immobilized Cibacron Blue 3GA. The purified enzyme had a mol.wt. of 43 000 and had an absorption spectrum characteristic of flavoprotein. The enzyme activity was enhanced by FMN and by CN-. The enzyme was inhibited by EDTA and by rho-chloromercuribenzoic acid.

Entities: Chemical Species

Mesh：

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Year: 1980 PMID： 7236205 PMCID： PMC1162236 DOI： 10.1042/bj1910457

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

19 in total

1. Studies of a halophilic NADH dehydrogenase. II. Kinetic properties of the enzyme in relation to salt activation.

Authors: L I Hochstein
Journal: Biochim Biophys Acta Date: 1975-09-22

Review 2. The DPNH dehydrogenase of the mitochondrial respiratory chain.

Authors: T P Singer; M Gutman
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1971

3. Comparative thermostability of enzymes from Bacillus stearothermophilus and Bacillus cereus.

Authors: R Amelunxen; M Lins
Journal: Arch Biochem Biophys Date: 1968-06 Impact factor: 4.013

4. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

5. Membranes of Bacillus stearothermophilus: factors affecting protoplast stability and thermostability of alkaline phosphatase and reduced nicotinamide adenine dinucleotide oxidase.

Authors: C Wisdom; N E Welker
Journal: J Bacteriol Date: 1973-06 Impact factor: 3.490

6. Studies of a halophilic NADH dehydrogenase. I. Purification and properties of the enzyme.

Authors: L I Hochstein; B P Dalton
Journal: Biochim Biophys Acta Date: 1973-04-12

7. Flavodoxin: a flavoprotein with ferredoxin activity from Clostrium pasteurianum.

Authors: E Knight; A J D'Eustachio; R W Hardy
Journal: Biochim Biophys Acta Date: 1966-03-07

8. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Authors: P Andrews
Journal: Biochem J Date: 1965-09 Impact factor: 3.857

9. Formation and repair of papain sulfenic acid.

Authors: W S Lin; D A Armstrong; G M Gaucher
Journal: Can J Biochem Date: 1975-03

10. A 3(17)beta-hydroxysteroid dehydrogenase in raterythrocytes. Conversion of 5alpha-dihydrotestosterone into 5alpha-androstane-3beta,17beta-diol and purification of the enzyme by affinity chromatography.

Authors: W Heyns; P De Moor
Journal: Biochim Biophys Acta Date: 1974-07-17

6 in total

Purification of an NADH-(dichlorophenol-indophenol) oxidoreductase from Bacillus stearothermophilus.

1. Studies of a halophilic NADH dehydrogenase. II. Kinetic properties of the enzyme in relation to salt activation.

Review 2. The DPNH dehydrogenase of the mitochondrial respiratory chain.

3. Comparative thermostability of enzymes from Bacillus stearothermophilus and Bacillus cereus.

4. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

5. Membranes of Bacillus stearothermophilus: factors affecting protoplast stability and thermostability of alkaline phosphatase and reduced nicotinamide adenine dinucleotide oxidase.

6. Studies of a halophilic NADH dehydrogenase. I. Purification and properties of the enzyme.

7. Flavodoxin: a flavoprotein with ferredoxin activity from Clostrium pasteurianum.

8. The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

9. Formation and repair of papain sulfenic acid.

10. A 3(17)beta-hydroxysteroid dehydrogenase in raterythrocytes. Conversion of 5alpha-dihydrotestosterone into 5alpha-androstane-3beta,17beta-diol and purification of the enzyme by affinity chromatography.

Review 1. Bacterial NADH-quinone oxidoreductases.

2. Separation Procedure and Partial Characterization of Two NAD(P)H Dehydrogenases from Cauliflower Mitochondria.

3. Isolated Diaphorase From Bovine Erythrocyte Cannot Reduce Oxidized Cytoglobin (Metcygb).

4. A soluble NADH dehydrogenase (NADH: ferricyanide oxidoreductase) from Thermus aquaticus strain T351.

5. Immunochemical probing of the structure and cofactor of NADH dehydrogenase from Paracoccus denitrificans.

6. A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis.