Literature DB >> 7226491

Improved colorimetric assay for glycosylated hemoglobin.

K M Parker, J D England, J Da Costa, R L Hess, D E Goldstein.   

Abstract

This colorimetric assay for glycosylated hemoglobin can be performed in 120 min with equipment available in most clinical laboratories. The glucose moiety of glycosylated hemoglobin is converted to 5-hydroxymethylfurfural by heating with oxalic acid for 60 min is an autoclave at 124 degrees C and 124 kPa (18 lb/in.2). The adduct formed by reacting 2-thiobarbituric acid with hydroxymethylfurfural is measured photometrically and results are expressed either as nanomoles of hydroxymethylfurfural or as fructose equivalents. Within-assay and between-assay coefficients of variation were less than 2% and less than 3%, respectively. Comparison of results for 50 patients' specimens as measured by the present assay and as analyzed for hemoglobin A1c by liquid chromatography showed excellent correlation (r = 0.98).

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Year:  1981        PMID： 7226491

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  31 in total

1.  Alpha-lipoic acid preserves the structural and functional integrity of red blood cells by adjusting the redox disturbance and decreasing O-GlcNAc modifications of antioxidant enzymes and heat shock proteins in diabetic rats.

Authors:  Mihailović Mirjana; Arambašić Jelena; Uskoković Aleksandra; Dinić Svetlana; Grdović Nevena; Marković Jelena; Poznanović Goran; Vidaković Melita
Journal:  Eur J Nutr       Date:  2011-11-18       Impact factor: 5.614

2.  Altered tissue content of trace metals in diabetic hyperinsulinaemic sand rats (Psammomys obesus).

Authors:  I Raz; J H Adler; E Havivi
Journal:  Diabetologia       Date:  1988-05       Impact factor: 10.122

3.  HbA1c standardisation destination--global IFCC Standardisation. How, why, where and when--a tortuous pathway from kit manufacturers, via inter-laboratory lyophilized and whole blood comparisons to designated national comparison schemes.

Authors:  Ian Goodall
Journal:  Clin Biochem Rev       Date:  2005-02

4.  A comparative evaluation of glycosylated haemoglobin assays: feasibility of references and standards.

Authors:  C M Peterson; L Jovanovic; P Raskin; D E Goldstein
Journal:  Diabetologia       Date:  1984-03       Impact factor: 10.122

5.  Glycosylation of hair: possible measure of chronic hyperglycaemia.

Authors:  R B Paisey; J R Clamp; M J Kent; N D Light; M Hopton; M Hartog
Journal:  Br Med J (Clin Res Ed)       Date:  1984-03-03

6.  Non-enzymatic glycosylation of skin collagen in patients with type 1 (insulin-dependent) diabetes mellitus and limited joint mobility.

Authors:  T J Lyons; L Kennedy
Journal:  Diabetologia       Date:  1985-01       Impact factor: 10.122

7.  Alpha-lipoic acid upregulates antioxidant enzyme gene expression and enzymatic activity in diabetic rat kidneys through an O-GlcNAc-dependent mechanism.

Authors:  Jelena Arambašić; Mirjana Mihailović; Aleksandra Uskoković; Svetlana Dinić; Nevena Grdović; Jelena Marković; Goran Poznanović; Djordje Bajec; Melita Vidaković
Journal:  Eur J Nutr       Date:  2012-10-12       Impact factor: 5.614

8.  Vanadyl sulfate administration protects the streptozotocin-induced oxidative damage to brain tissue in rats.

Authors:  Refiye Yanardag; Sevim Tunali
Journal:  Mol Cell Biochem       Date:  2006-03-11       Impact factor: 3.396

9.  A study of the antidiabetic activity of Barleria prionitisLinn.

Authors:  Reema Dheer; Pradeep Bhatnagar
Journal:  Indian J Pharmacol       Date:  2010-04       Impact factor: 1.200

10.  Relationship of patient request fulfillment to compliance, glycemic control, and other health care outcomes in insulin-dependent diabetes.

Authors:  R F Uhlmann; T S Inui; R E Pecoraro; W B Carter
Journal:  J Gen Intern Med       Date:  1988 Sep-Oct       Impact factor: 5.128
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