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Literature DB >> 7220346

IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.

Abstract

Analysis of primary transcripts made by Escherichia coli RNA polymerase on T4 DNA containing an intact or partially deleted tRNA gene cluster demonstrates that the T4 tRNA genes are transcribed from two promoters differing in their strength. The stronger (P1) and the weaker (P2) promoters are located at distances of 1 kb and 1.5 kb from the tRNA genes, respectively. Selective initiation of individual transcripts with dinucleotides shows that P1 and P2 promoters contain the sequences TAT and CAC respectively. The two-promoter organisation of the tRNA cluster may reflect two superimposed mechanisms of gene expression in T4-infected bacteria.

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Year: 1981 PMID： 7220346 PMCID： PMC327219 DOI： 10.1093/nar/9.3.519

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

4. Host--virus interactions in the control of T4 prereplicative transcription. II. Interaction between tabC (rho) mutants and T4 mot mutants.

Authors: J F Pulitzer; A Coppo; M Caruso
Journal: J Mol Biol Date: 1979-12-25 Impact factor: 5.469

5. Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4.

Authors: A Goldfarb; H J Burger
Journal: Nucleic Acids Res Date: 1981-06-25 Impact factor: 16.971

5 in total

IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.

1. Initiation and transcription of a set of transfer ribonucleic acid genes in vitro.

2. Bacteriophage T4 transfer RNA. 3. Clustering of the genes for the T4 transfer RNA's.

3. Bacteriophage T4 gene expression. Evidence for two classes of prereplicative cistrons.

4. Host--virus interactions in the control of T4 prereplicative transcription. II. Interaction between tabC (rho) mutants and T4 mot mutants.

5. A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.

6. A restriction map of the T4 transfer RNA gene cluster.

7. The cloning of a T4 transfer RNA gene cluster.

8. Isolation and characterization of conditional lethal mutations in the mot gene of bacteriophage T4.

9. In vitro transcription and isolation of a polycistronic RNA product of the T4 tRNA operon.

10. Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides.

1. A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro.

2. T4-induced antipolarity: temporal heterogeneity in response of early transcription units.

3. Control of promoter utilization by bacteriophage T4-induced modification of RNA polymerase alpha subunit.

4. Changes in the promoter range of RNA polymerase resulting from bacteriophage T4-induced modification of core enzyme.

5. Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4.