Literature DB >> 668702

Transcription of bacteriophage T4 DNA in vitro: selective initiation with dinucleotides.

W Rüger.   

Abstract

The transcription products of phage T4 DNA in vitro are separated on polyacrylamide gels. The influence of salt, polymerase, triphosphate concentration and glucosylation on the RNA synthesis are shown. Individual transcripts are initiated selectively with dinucleotides and a single triphosphate. This technique allows the prediction of the initiation sequences of several T4 transcripts.

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Year:  1978        PMID： 668702     DOI： 10.1111/j.1432-1033.1978.tb12427.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  11 in total

1.  T4-induced alpha- and beta-glucosyltransferase: cloning of the genes and a comparison of their products based on sequencing data.

Authors:  J Tomaschewski; H Gram; J W Crabb; W Rüger
Journal:  Nucleic Acids Res       Date:  1985-11-11       Impact factor: 16.971

2.  Physical mapping of bacteriophage T4.

Authors:  E Niggemann; I Green; H P Meyer; W Rüger
Journal:  Mol Gen Genet       Date:  1981

3.  A physical map of bacteriophage T4 including the positions of strong promoters and terminators recognized in vitro.

Authors:  H Gram; H D Liebig; A Hack; E Niggemann; W Rüger
Journal:  Mol Gen Genet       Date:  1984

4.  Physical mapping of the restriction fragments obtained from bacteriophage T4 dC-DNA with the restriction endonucleases SmaI, KpnI and BglII.

Authors:  H Kiko; E Niggemann; W Rüger
Journal:  Mol Gen Genet       Date:  1979

5.  Changes in the promoter range of RNA polymerase resulting from bacteriophage T4-induced modification of core enzyme.

Authors:  A Goldfarb
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

6.  Mapping of in vitro transcription units and identification of primary transcripts of the D region of bacteriophage T4.

Authors:  A Goldfarb; H J Burger
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

7.  IN vitro transcription of bacteriophage T4 tRNA gene cluster from two different promoters.

Authors:  A Goldfarb
Journal:  Nucleic Acids Res       Date:  1981-02-11       Impact factor: 16.971

8.  Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex.

Authors:  H K Song; S H Sohn; S W Suh
Journal:  EMBO J       Date:  1999-03-01       Impact factor: 11.598

9.  The region of phage T4 genes 34, 33 and 59: primary structures and organization on the genome.

Authors:  S Hahn; U Kruse; W Rüger
Journal:  Nucleic Acids Res       Date:  1986-12-09       Impact factor: 16.971

10.  Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose.

Authors:  A Vrielink; W Rüger; H P Driessen; P S Freemont
Journal:  EMBO J       Date:  1994-08-01       Impact factor: 11.598
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