Literature DB >> 7207472

Selective removal of histone H1 from nucleosomes at low ionic strength.

S P Modak, J J Lawrence, C Gorka.   

Abstract

The method for removal of histone H 1 from chromatin by treatment with ion-exchange resin AG 50 WX 2 in the presence of 100 mM NaCl and 50 mM phosphate buffer (Thoma and Koller, 1977, Cell, 12, 101-107) results in production not only of H1-depleted chromatin but also free DNA. We have not modified this procedure so that the nucleosome is treated with the cation exchange resin in two steps, first in 50 mM sodium phosphate buffer and then in 50 mM sodium phosphate and 50 mM NaCl whereby histone H 1 is selectively removed without a release of free DNA at low resin concentrations.

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Year:  1980        PMID： 7207472     DOI： 10.1007/bf00777531

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  20 in total

1.  Heterogeneity of chromatin subunits in vitro and location of histone H1.

Authors:  A J Varshavsky; V V Bakayev; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971

2.  Removal of histone H1 exposes a fifty base pair DNA segment between nucleosomes.

Authors:  J P Whitlock; R T Simpson
Journal:  Biochemistry       Date:  1976-07-27       Impact factor: 3.162

3.  DNA degradation in terminally differentiating lens fiber cells from chick embryos.

Authors:  D W Appleby; S P Modak
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

4.  Selective displacement of histone H1 from whole HeLa nuclei: effect on chromatin structure in situ as probed by micrococcal nuclease.

Authors:  G M Lawson; R D Cole
Journal:  Biochemistry       Date:  1979-05-29       Impact factor: 3.162

5.  Selective dissociation of histones from calf thymus nucleoprotein.

Authors:  H H Ohlenbusch; B M Olivera; D Tuan; N Davidson
Journal:  J Mol Biol       Date:  1967-04-28       Impact factor: 5.469

6.  Studies on deoxyribonucleoprotein structure. Redistribution of proteins in mixtures of deoxyribonucleoproteins, DNA and RNA.

Authors:  Y V Ilyin; A Y Varshavsky; U N Mickelsaar; G P Georgiev
Journal:  Eur J Biochem       Date:  1971-09-24

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Action of micrococcal nuclease on chromatin and the location of histone H1.

Authors:  M Noll; R D Kornberg
Journal:  J Mol Biol       Date:  1977-01-25       Impact factor: 5.469

9.  Reversible dissociation of linker histone from chromatin with preservation of internucleosomal repeat.

Authors:  J Allan; D Z Staynov; H Gould
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

10.  Histone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.

Authors:  S Muyldermans; I Lasters; L Wyns
Journal:  Nucleic Acids Res       Date:  1980-02-25       Impact factor: 16.971
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  4 in total

1.  Chromatin prepared using micrococcal nuclease retains the enzyme activity which can be removed with cation-exchange resin AG 50 WX2.

Authors:  S B Goel; S P Modak
Journal:  Nucleic Acids Res       Date:  1984-02-10       Impact factor: 16.971

2.  Involvement of histone H1 in the structure of the linker DNA in nucleosomes as revealed by nucleases.

Authors:  J J Lawrence; P Goeltz
Journal:  Mol Biol Rep       Date:  1981-11-30       Impact factor: 2.316

3.  The involvement of histone H1[0] in chromatin structure.

Authors:  J Roche; J L Girardet; C Gorka; J J Lawrence
Journal:  Nucleic Acids Res       Date:  1985-04-25       Impact factor: 16.971

4.  The structural role of histone H1: properties of reconstituted chromatin with various H1 subfractions (H1-1, H1-2, and H1o).

Authors:  J Biard-Roche; C Gorka; J J Lawrence
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
  4 in total

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