Literature DB >> 7193228

Enzyme-linked immunosorbent assay for screening monoclonal antibody production: use of intact cells as antigen.

J Y Douillard, T Hoffman, R B Herberman.   

Abstract

An enzyme-linked immunosorbent assay (ELIZA) was developed for screening production of monoclonal antibodies with specificity for surface membrane components on human mononuclear cells. Whole cells used as antigen were desiccated under vacuum in flexible polyvinyl chloride plates or in rigid plates coated with protein-detergent solution. Rabbit or goat anti-mouse IgG conjugated with peroxidase was used as indicator after affinity column purification. Under these conditions, the sensitivity of ELISA proved comparable to other binding techniques or microcytoxicity and allowed rapid, reproducible, and efficient detection of antibody producing hybridomas.

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Year:  1980        PMID： 7193228     DOI： 10.1016/0022-1759(80)90231-8

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  9 in total

1.  Inhibition of HLA class I antigen and mRNA expression induced by Rous sarcoma virus in transformed human fibroblasts.

Authors:  J Gogusev; B Teutsch; M T Morin; F Mongiat; F Haguenau; G Suskind; G F Rabotti
Journal:  Proc Natl Acad Sci U S A       Date:  1988-01       Impact factor: 11.205

2.  A simple and sensitive solid-phase radioimmunoassay for the assay of human TSH antibody. Use in screening hybridoma cell lines.

Authors:  S Iwasa; S Pincus; E Saito; N P Laborde; W D Odell
Journal:  Appl Biochem Biotechnol       Date:  1983-08       Impact factor: 2.926

3.  Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

Authors:  J Morrison-Plummer; J F Alderete; J B Baseman
Journal:  Br J Vener Dis       Date:  1983-04

4.  An automatic fluorescence micro-ELISA system for quantitative screening of hybridoma supernatants using a protein-A-beta-galactosidase conjugate.

Authors:  P L Van Soest; J de Josselin de Jong; P M Lansdorp; W Van Ewijk
Journal:  Histochem J       Date:  1984-01

5.  A solid-phase radioimmunoassay to detect antibodies produced by hybridomas to antigens derived from human melanoma cells.

Authors:  P J Kelleher; H L Mathews; L K Woods; R S Farr; P Minden
Journal:  Cancer Immunol Immunother       Date:  1983       Impact factor: 6.968

6.  Monoclonal antibody against ependymoma-derived cell line.

Authors:  Y Mihara; Y Matsukado; S Goto; Y Ushio; S Tokumitsu; K Takahashi
Journal:  J Neurooncol       Date:  1992-01       Impact factor: 4.130

7.  Stimulation of human monocytes by anti-CD3 monoclonal antibody: induction of inflammatory mediator release via immobilization of Fc receptor by adsorbed immunoglobulin and T-lymphocytes.

Authors:  T Hoffman; A K Tripathi; Y L Lee; E F Lizzio; E Bonvini
Journal:  Inflammation       Date:  1992-12       Impact factor: 4.092

8.  Anti-tumor antibody produced by human tumor-infiltrating and peripheral blood B lymphocytes.

Authors:  C J Punt; J A Barbuto; H Zhang; W J Grimes; K D Hatch; E M Hersh
Journal:  Cancer Immunol Immunother       Date:  1994-04       Impact factor: 6.968

9.  Diagnostic accuracy of specific IgG antibodies for bird fancier's lung: a systematic review and meta-analysis.

Authors:  Akihiro Shiroshita; Yu Tanaka; Kei Nakashima; Yuki Furukawa; Yuki Kataoka
Journal:  Ann Transl Med       Date:  2019-11
  9 in total

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