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Literature DB >> 6339000

Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

J Morrison-Plummer, J F Alderete, J B Baseman.

Abstract

A highly sensitive enzyme-linked immunosorbent assay was used for the analysis of serum IgG reactivity against specific immunogenic Treponema pallidum proteins. Outer membrane treponemal proteins purified by preparative SDS-polyacrylamide gel electrophoresis were used as antigenic probes at concentrations as low as 100 ng per ml (5 ng per well in microtitre plates). Detection of anti-treponemal antibody was possible using rabbit syphilitic sera diluted to 1/10 000. The sensitivity of the assay was equal to or greater than that detected by radioimmuno-precipitation combined with gel electrophoresis and fluorography techniques and was capable of monitoring host IgG responses throughout the progress of the disease.

Entities: Chemical Species

Mesh：

Substances：

Year: 1983 PMID： 6339000 PMCID： PMC1046140 DOI： 10.1136/sti.59.2.75

Source DB: PubMed Journal: Br J Vener Dis ISSN： 0007-134X

17 in total

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

2. Detection of antibodies to Mycoplasma pulmonis by an enzyme-linked immunosorbent assay.

Authors: S A Horowitz; G H Cassell
Journal: Infect Immun Date: 1978-10 Impact factor: 3.441

3. The use of freeze-preserved treponemes in the Treponema pallidum immobilization test.

Authors: E E Nell; P H Hardy
Journal: Cryobiology Date: 1972-10 Impact factor: 2.487

4. Biologic false-positive reactions for syphilis as measured by the Treponema pallidum immobilization test.

Authors: R A Le Clair; T S Montague; L M Keetin
Journal: J Med Date: 1972

5. Chronic biological false-positive reactions to serological tests for syphilis in blood donors.

Authors: M F Garner; J L Backhouse
Journal: J Clin Pathol Date: 1970-09 Impact factor: 3.411

6. Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis.

Authors: T Rathlev
Journal: Br J Vener Dis Date: 1967-09

7. Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus.

Authors: J T Roehrig; J A Corser; M J Schlesinger
Journal: Virology Date: 1980-02 Impact factor: 3.616

8. Surface-associated host proteins on virulent Treponema pallidum.

Authors: J F Alderete; J B Baseman
Journal: Infect Immun Date: 1979-12 Impact factor: 3.441

9. Problems with beaded fluorescence pattern in FTA-ABS test.

Authors: F D Pien; H Markowitz; C H McKenna; A L Schroeter
Journal: J Am Vener Dis Assoc Date: 1976-09

10. Microassay for immunoglobulin G antibodies to Treponema pallidum with radioiodinated protein A from staphylococcus aureus: immunoglobulin G response in experimental syphilis in rabbits.

Authors: P M Zeltzer; J S Pepose; N H Bishop; J N Miller
Journal: Infect Immun Date: 1978-07 Impact factor: 3.441

9 in total

4. Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: use of whole cells and aqueous extract as antigen.

Authors: J F Alderete
Journal: Br J Vener Dis Date: 1984-06

Review 9. Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

Authors: S J Norris
Journal: Microbiol Rev Date: 1993-09

9 in total

Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum.

1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

2. Detection of antibodies to Mycoplasma pulmonis by an enzyme-linked immunosorbent assay.

3. The use of freeze-preserved treponemes in the Treponema pallidum immobilization test.

4. Biologic false-positive reactions for syphilis as measured by the Treponema pallidum immobilization test.

5. Chronic biological false-positive reactions to serological tests for syphilis in blood donors.

6. Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis.

7. Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus.

8. Surface-associated host proteins on virulent Treponema pallidum.

9. Problems with beaded fluorescence pattern in FTA-ABS test.

10. Microassay for immunoglobulin G antibodies to Treponema pallidum with radioiodinated protein A from staphylococcus aureus: immunoglobulin G response in experimental syphilis in rabbits.

1. Comparison of major protein antigens and protein profiles of Treponema pallidum and Treponema pertenue.

2. Monoclonal antibodies to Treponema pallidum: recognition of a major polypeptide antigen.

3. Detection of immunoglobulin M in cerebrospinal fluid from syphilis patients by enzyme-linked immunosorbent assay.

4. Enzyme linked immunosorbent assay for detecting antibody to Trichomonas vaginalis: use of whole cells and aqueous extract as antigen.

5. Radioimmunoassays for the detection of antibodies to treponemal polypeptide antigens in serum.

6. Enumeration of Treponema pallidum cells cultivated in vitro by an enzyme-linked immunosorbent assay.

7. Ig class and IgG subclass responses to Treponema pallidum in patients with syphilis.

8. Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli.

Review 9. Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.