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Literature DB >> 7186939

Differentiation between serum stimulation of ouabain-resistant and sensitive Rb influx in quiescent NIH 3T3 cells.

Abstract

The addition of serum to quiescent NIH 3T3 mouse cell cultures resulted in a 10- to 20-fold increase of Rb influx which was resistant to ouabain, and only a three- to fourfold activation of ouabain-sensitive Rb influx. Stimulation of the ouabain-resistant Rb influx following serum addition reached its maximum within 2 min. The stimulation of ouabain-resistant Rb influx was a result of Vm increase while the Km for Rb was unchanged. Ouabain-resistant Rb influx, after serum addition, was resistant to amiloride and sensitive to ethacrynic acid. Replacing chloride in the medium by NO3-, CO3- and CH3COO- resulted in a drastic decrease in the ouabain-resistant Rb influx. It appeared, therefore, that the ouabain-resistant Rb influx in NIH 3T3 cells was Cl--dependent.

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Year: 1982 PMID： 7186939 DOI： 10.1007/bf01870226

Source DB: PubMed Journal: J Membr Biol ISSN： 0022-2631 Impact factor: 1.843

23 in total

Review 1. Control of growth of mammalian cells in cell culture.

Authors: R W Holley
Journal: Nature Date: 1975-12-11 Impact factor: 49.962

2. Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells.

Authors: E Rozengurt; L A Heppel
Journal: Proc Natl Acad Sci U S A Date: 1975-11 Impact factor: 11.205

3. Potassium transport and content during G1 and S phase following serum stimulation of 3T3 cells.

Authors: J T Tupper; F Zorgniotti; B Mills
Journal: J Cell Physiol Date: 1977-06 Impact factor: 6.384

4. Mode of action of amiloride in toad urinary bladder. An electrophysiological study of the drug action on sodium permeability of the mucosal border.

Authors: K Sudou; T Hoshi
Journal: J Membr Biol Date: 1977-04-07 Impact factor: 1.843

5. Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry.

Authors: J B Smith; E Rozengurt
Journal: Proc Natl Acad Sci U S A Date: 1978-11 Impact factor: 11.205

Review 6. Monovalent ion fluxes and the control of cell proliferation in cultured fibroblasts.

Authors: E Rozengurt; S Mendoza
Journal: Ann N Y Acad Sci Date: 1980 Impact factor: 5.691

7. A chloride dependent K+ flux induced by N-ethylmaleimide in genetically low K+ sheep and goat erythrocytes.

Authors: P K Lauf; B E Theg
Journal: Biochem Biophys Res Commun Date: 1980-02-27 Impact factor: 3.575

8. Lithium transport by fibroblastic mouse cells: characterization and stimulation by serum and growth factors in quiescent cultures.

Authors: J B Smith; E Rozengurt
Journal: J Cell Physiol Date: 1978-12 Impact factor: 6.384

6. Amiloride added together with bumetanide completely blocks mouse 3T3-cell exit from G0/G1-phase and entry into S-phase.

Authors: R Panet; D Snyder; H Atlan
Journal: Biochem J Date: 1986-11-01 Impact factor: 3.857

7. Rb+ influxes differentiate between growth arrest of cells by different agents.

Authors: R Panet; I Fromer; A Alayoff
Journal: J Membr Biol Date: 1983 Impact factor: 1.843

8. Stimulation of bumetanide-sensitive Na+/K+/Cl- cotransport by different mitogens in synchronized human skin fibroblasts is essential for cell proliferation.

Authors: R Panet; H Atlan
Journal: J Cell Biol Date: 1991-07 Impact factor: 10.539

8 in total

Differentiation between serum stimulation of ouabain-resistant and sensitive Rb influx in quiescent NIH 3T3 cells.

Review 1. Control of growth of mammalian cells in cell culture.

2. Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells.

3. Potassium transport and content during G1 and S phase following serum stimulation of 3T3 cells.

4. Mode of action of amiloride in toad urinary bladder. An electrophysiological study of the drug action on sodium permeability of the mucosal border.

5. Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry.

Review 6. Monovalent ion fluxes and the control of cell proliferation in cultured fibroblasts.

7. A chloride dependent K+ flux induced by N-ethylmaleimide in genetically low K+ sheep and goat erythrocytes.

8. Lithium transport by fibroblastic mouse cells: characterization and stimulation by serum and growth factors in quiescent cultures.

9. Effect of anions of potassium self-exchange in ascites tumor cells.

10. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.

1. Emerging roles for sodium dependent amino acid transport in mesenchymal cells.

2. Protective effect of eicosapentaenoic acid on ouabain toxicity in neonatal rat cardiac myocytes.

3. Positive inotropic effects of the endogenous Na+/K(+)-transporting ATPase inhibitor from the hypothalamus.

4. Ouabain-resistant Na+, K+ transport system in mouse NIH 3T3 cells.

5. Nitrogen mustard interference with potassium transport systems in Ehrlich ascites tumor cells.

6. Amiloride added together with bumetanide completely blocks mouse 3T3-cell exit from G0/G1-phase and entry into S-phase.

7. Rb+ influxes differentiate between growth arrest of cells by different agents.

8. Stimulation of bumetanide-sensitive Na+/K+/Cl- cotransport by different mitogens in synchronized human skin fibroblasts is essential for cell proliferation.