The energetics of D-fucose transport in Saccharomyces fragilis. The influence of the protonmotive force on sugar accumulation.

The protonmotive force in Saccharomyces fragilis has been estimated under various experimental conditions. The transmembrane potential has been monitored with tetraphenylphosphonium and 3,3'-dipropylthiadicarbocyanine. The distribution ratio of these cations between intracellular and extracellular water appeared to be governed by the electrical potential difference across the membrane of this yeast strain. The transmembrane pH difference was deduced from dimethyloxazolidinedione uptake experiments and from direct measurements of intracellular pH after freezing and boiling of the cells. Both methods yielded similar results. D-Fucose is transported by S. fragilis via H+ symport, with a H+/fucose stoichiometry of approximately 1. Accumulation of this sugar appeared to be closely correlated with the protonmotive force.