Literature DB >> 7161566

A modified procedure for thin-layer chromatography of phospholipids.

D Allan, S Cockcroft.   

Abstract

We have found that when lipid samples from a variety of tissues are run on EDTA-impregnated plates in a solvent system which contains a reduced amount of acetic acid and water compared with that used by Skipski et al. (1964. Biochem. J. 90: 374-378), there is a clear separation among sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine, and phosphatidate. This modified procedure appears to offer significant advantages over the original method described by Skipski et al. particularly for studies of the metabolism of anionic phospholipids.

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Year:  1982        PMID： 7161566

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  11 in total

1.  Thin-layer chromatography of phospholipids.

Authors:  Rania M Deranieh; Amit S Joshi; Miriam L Greenberg
Journal:  Methods Mol Biol       Date:  2013

2.  Protein and diacylglycerol phosphorylation in the stimulus-secretion coupling of rat mast cells.

Authors:  R Sagi-Eisenberg; J C Foreman; P J Raval; S Cockcroft
Journal:  Immunology       Date:  1987-06       Impact factor: 7.397

3.  Protein and lipid fingerprinting of native-like membrane complexes by combining TLC and protein electrophoresis.

Authors:  Elena Lopez-Rodriguez; Nuria Roldan; Begoña Garcia-Alvarez; Jesús Pérez-Gil
Journal:  J Lipid Res       Date:  2018-11-21       Impact factor: 5.922

4.  Relationship between arachidonate generation and exocytosis in permeabilized mast cells.

Authors:  Y Churcher; D Allan; B D Gomperts
Journal:  Biochem J       Date:  1990-02-15       Impact factor: 3.857

5.  The N-terminal region of acyl-CoA synthetase 3 is essential for both the localization on lipid droplets and the function in fatty acid uptake.

Authors:  Margarete Poppelreuther; Berenice Rudolph; Chen Du; Regina Großmann; Melanie Becker; Christoph Thiele; Robert Ehehalt; Joachim Füllekrug
Journal:  J Lipid Res       Date:  2012-02-22       Impact factor: 5.922

6.  The receptors for ATP and fMetLeuPhe are independently coupled to phospholipases C and A2 via G-protein(s). Relationship between phospholipase C and A2 activation and exocytosis in HL60 cells and human neutrophils.

Authors:  S Cockcroft; J Stutchfield
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857

Review 7.  Lipidomic analysis of cerebrospinal fluid by mass spectrometry-based methods.

Authors:  Benoit Colsch; Alexandre Seyer; Samia Boudah; Christophe Junot
Journal:  J Inherit Metab Dis       Date:  2014-12-09       Impact factor: 4.982

8.  Identification of phospholipase B from Dictyostelium discoideum reveals a new lipase family present in mammals, flies and nematodes, but not yeast.

Authors:  Clive P Morgan; Robert Insall; Lee Haynes; Shamshad Cockcroft
Journal:  Biochem J       Date:  2004-09-01       Impact factor: 3.857

9.  Ca2+-induced polyphosphoinositide breakdown due to phosphomonoesterase activity in chicken erythrocytes.

Authors:  P J Raval; D Allan
Journal:  Biochem J       Date:  1985-10-01       Impact factor: 3.857

10.  Alternation in the glycolipid transfer protein expression causes changes in the cellular lipidome.

Authors:  Matti A Kjellberg; Anders P E Backman; Henna Ohvo-Rekilä; Peter Mattjus
Journal:  PLoS One       Date:  2014-05-13       Impact factor: 3.240
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