Literature DB >> 7150237

A simple test for macromolecular heterogeneity in the analytical ultracentrifuge.

J M Creeth, S E Harding.   

Abstract

A simple check for the presence of heterogeneity in a macromolecular system is proposed, employing comparison of Rayleigh sedimentation-equilibrium patterns for two solutions of the same fringe concentration but differing absolute concentrations. The method is illustrated by application to a bronchial glycoprotein from a cystic-fibrosis patient.

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Year:  1982        PMID： 7150237      PMCID： PMC1158533          DOI： 10.1042/bj2050639

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  3 in total

1.  EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.

Authors:  D A YPHANTIS
Journal:  Biochemistry       Date:  1964-03       Impact factor: 3.162

2.  Characterization of proteins by sedimentation equilibrium in the analytical ultracentrifuge.

Authors:  D C Teller
Journal:  Methods Enzymol       Date:  1973       Impact factor: 1.600

3.  Detection in the ultracentrifuge of protein heterogeneity by computer modelling, illustrated by pyruvate dehydrogenase multienzyme complex.

Authors:  G A Gilbert; L M Gilbert
Journal:  J Mol Biol       Date:  1980-12-15       Impact factor: 5.469
  3 in total
  5 in total

1.  Adenosylcobalamin-dependent glutamate mutase: properties of a fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit.

Authors:  D E Holloway; S E Harding; E N Marsh
Journal:  Biochem J       Date:  1996-12-15       Impact factor: 3.857

2.  An analysis of the heterogeneity of mucins. No evidence for a self-association.

Authors:  S E Harding
Journal:  Biochem J       Date:  1984-05-01       Impact factor: 3.857

3.  Biophysical Reviews' "meet the editors series"-a profile of Steve Harding's career in macromolecular hydrodynamics.

Authors:  Stephen E Harding
Journal:  Biophys Rev       Date:  2022-06-25

4.  Physicochemical studies on turnip-yellow-mosaic virus. Homogeneity, relative molecular masses, hydrodynamic radii and concentration-dependence of parameters in non-dissociating solvents.

Authors:  S E Harding; P Johnson
Journal:  Biochem J       Date:  1985-11-01       Impact factor: 3.857

Review 5.  Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis.

Authors:  Stephen E Harding; Richard B Gillis; Gary G Adams
Journal:  Biophys Rev       Date:  2016-11-22
  5 in total

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