Literature DB >> 7140719

Uroporphyrinogen decarboxylase. A method for measuring enzyme activity.

J G Straka, J P Kushner, M A Pryor.   

Abstract

A method for measuring the activity of uroporphyrinogen decarboxylase from a variety of sources is described. Porphyrinogen substrates are generated by enzymic synthesis or chemical reduction. Substrates and enzyme are incubated under standardized conditions. The reactions are then stopped and the reaction products, as well as unmetabolized substrate, are absorbed on tale, eluted and esterified. Porphyrin esters are then separated and quantified using high performance liquid chromatography.

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Year:  1982        PMID： 7140719

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  6 in total

1.  Steady-state levels of uroporphyrinogen decarboxylase mRNA in lymphoblastoid cell lines from patients with familial porphyria cutanea tarda and their relatives.

Authors:  J L Hansen; M A Pryor; J B Kennedy; J P Kushner
Journal:  Am J Hum Genet       Date:  1988-06       Impact factor: 11.025

2.  Crystal structure of human uroporphyrinogen decarboxylase.

Authors:  F G Whitby; J D Phillips; J P Kushner; C P Hill
Journal:  EMBO J       Date:  1998-05-01       Impact factor: 11.598

3.  Characterization and crystallization of human uroporphyrinogen decarboxylase.

Authors:  J D Phillips; F G Whitby; J P Kushner; C P Hill
Journal:  Protein Sci       Date:  1997-06       Impact factor: 6.725

4.  Uroporphyrinogen decarboxylase: a splice site mutation causes the deletion of exon 6 in multiple families with porphyria cutanea tarda.

Authors:  J R Garey; L M Harrison; K F Franklin; K M Metcalf; E S Radisky; J P Kushner
Journal:  J Clin Invest       Date:  1990-11       Impact factor: 14.808

5.  Modified uroporphyrinogen decarboxylase activity in a yeast mutant which mimics porphyria cutanea tarda.

Authors:  J Rytka; T Bilinski; R Labbe-Bois
Journal:  Biochem J       Date:  1984-03-01       Impact factor: 3.857

6.  Reduction of porphyrins to porphyrinogens with palladium on carbon.

Authors:  Hector A Bergonia; John D Phillips; James P Kushner
Journal:  Anal Biochem       Date:  2008-09-23       Impact factor: 3.365
  6 in total

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