Literature DB >> 7140716

Delta-aminolevulinic acid dehydratase assay.

S Sassa.   

Abstract

gamma-Aminolevulinic acid (ALA) dehydratase catalyzes the synthesis of porphobilinogen (PBG) from two molecules of ALA. A semimicro method for the colorimetric determination of ALA dehydratase is presented and applied to various tissues. The enzyme activity in adult male rat liver was 2.22 and 1.94 mumol PBG formed/g liver/h for homogenates assayed with or without dithiothreitol, respectively. The assay was linear for at least 2.5 h and for up to 2.5 mg tissue per assay. The Km for ALA was 4.0 X 10(-4)M and the pH optimum was 6.2-6.4. The effects of activators and inhibitors on enzyme activity are discussed.

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Year:  1982        PMID： 7140716     DOI： 10.1159/000459097

Source DB:  PubMed          Journal:  Enzyme        ISSN： 0013-9432


  57 in total

1.  Direct assay of delta-aminolevulinic acid dehydratase in heme biosynthesis for the detection of porphyrias by tandem mass spectrometry.

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Journal:  Anal Chem       Date:  2010-08-01       Impact factor: 6.986

2.  Oxidative stress parameters in blood, liver, and kidney of diabetic rats treated with curcumin and/or insulin.

Authors:  Heloisa Einloft Palma; Patrícia Wolkmer; Miguel Gallio; Marcos M B Corrêa; Roberta Schmatz; Gustavo R Thomé; Luciane B Pereira; Verônica S P Castro; Andréia B Pereira; Andressa Bueno; Lizielle S de Oliveira; Debora Rosolen; Thaís R Mann; Bianca S de Cecco; Dominguita L Graça; Sonia T A Lopes; Cinthia M A Mazzanti
Journal:  Mol Cell Biochem       Date:  2013-10-16       Impact factor: 3.396

3.  ORY supplementation mitigates acetaminophen-induced acute liver failure in male mice: role of oxidative stress and apoptotic markers.

Authors:  Marcelo Gomes de Gomes; Lucian Del Fabbro; André Rossito Goes; Leandro Cattelan Souza; Silvana Peterini Boeira; Cristiano Ricardo Jesse
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2020-06-29       Impact factor: 3.000

4.  The effects of acute-phase inducers and dimethyl sulphoxide on delta-aminolaevulinate synthase activity in human HepG2 hepatoma cells.

Authors:  F Iwasa; S Sassa; A Kappas
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

5.  Induction of haem synthesis in Hep G2 human hepatoma cells by dimethyl sulphoxide. A transcriptionally activated event.

Authors:  R A Galbraith; S Sassa; A Kappas
Journal:  Biochem J       Date:  1986-07-15       Impact factor: 3.857

6.  Organoselenium bis selenide attenuates 3-nitropropionic acid-induced neurotoxicity in rats.

Authors:  Cristiani F Bortolatto; Cristiano R Jesse; Ethel A Wilhelm; Pietro M Chagas; Cristina W Nogueira
Journal:  Neurotox Res       Date:  2012-06-28       Impact factor: 3.911

7.  Gallic acid modulates cerebral oxidative stress conditions and activities of enzyme-dependent signaling systems in streptozotocin-treated rats.

Authors:  I J Kade; J B T Rocha
Journal:  Neurochem Res       Date:  2013-02-05       Impact factor: 3.996

8.  Comparative studies on dicholesteroyl diselenide and diphenyl diselenide as antioxidant agents and their effect on the activities of Na+/K+ ATPase and delta-aminolevulinic acid dehydratase in the rat brain.

Authors:  Ige J Kade; Marcio W Paixão; Oscar E D Rodrigues; Nilda B V Barbosa; Antonio L Braga; Daiana S Avila; Cristina W Nogueira; João B T Rocha
Journal:  Neurochem Res       Date:  2007-08-21       Impact factor: 3.996

9.  Effects of expression of hemA and hemB genes on production of porphyrin in Propionibacterium freudenreichii.

Authors:  Yongzhe Piao; Pornpimon Kiatpapan; Mitsuo Yamashita; Yoshikatsu Murooka
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

10.  Effects of diphenyl diselenide diet on a model of mercury poisoning.

Authors:  Tiago da Luz Fiuza; Jossiele Leitemperger; Eduardo Stringini Severo; Aline Teixeira Marins; Aline Blank do Amaral; Maria Ester Pereira; Vania Lucia Loro
Journal:  Mol Biol Rep       Date:  2018-10-23       Impact factor: 2.316
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