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Literature DB >> 7134807

A rapid one-step method for the isolation of human granulocytes from whole blood.

Abstract

A rapid one-step method for the separation of granulocytes, mononuclear cells and erythrocytes from peripheral blood using two different gradients of Percoll (density solution I: 1.075 kg/l; solution II: 1.096-1.098 kg/l) is described. The influence of temperature on the density of solution II was tested and the variations were found to be of some importance. The average yield of granulocytes from 14 donors was 87.6 +/- 9.6%, and the purity of the granulocytes was 99.66 +/- 0.33%.

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Year: 1982 PMID： 7134807

Source DB: PubMed Journal: Scand J Clin Lab Invest ISSN： 0036-5513 Impact factor: 1.713

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Cited

26 in total

1. A dual role for diacylglycerol kinase generated phosphatidic acid in autoantibody-induced neutrophil exocytosis.

Authors: Neil J Holden; Caroline O S Savage; Stephen P Young; Michael J Wakelam; Lorraine Harper; Julie M Williams
Journal: Mol Med Date: 2011-08-08 Impact factor: 6.354

2. A rapid method for the preparation of the neutrophil fraction of granulocytes from human blood by centrifugation on isotonic Nycodenz gradients.

Authors: T C Ford; R Needle; D Rickwood
Journal: Blut Date: 1987-06

3. Oligomeric coiled-coil adhesin YadA is a double-edged sword.

Authors: Salome Casutt-Meyer; Francesco Renzi; Mathias Schmaler; Naja J Jann; Marlise Amstutz; Guy R Cornelis
Journal: PLoS One Date: 2010-12-08 Impact factor: 3.240

4. Effect of fluticasone propionate on neutrophil chemotaxis, superoxide generation, and extracellular proteolytic activity in vitro.

Authors: C G Llewellyn-Jones; S L Hill; R A Stockley
Journal: Thorax Date: 1994-03 Impact factor: 9.139

9. Effects of recombinant GM-CSF and IgA opsonisation on neutrophil phagocytosis of latex beads coated with P6 outer membrane protein from Haemophilus influenzae.

Authors: D Burnett; A Chamba; R A Stockley; T F Murphy; S L Hill
Journal: Thorax Date: 1993-06 Impact factor: 9.139

10. Superoxide production by Crohn's disease neutrophils.

Authors: F T Curran; R N Allan; M R Keighley
Journal: Gut Date: 1991-04 Impact factor: 23.059

A rapid one-step method for the isolation of human granulocytes from whole blood.

1. A dual role for diacylglycerol kinase generated phosphatidic acid in autoantibody-induced neutrophil exocytosis.

2. A rapid method for the preparation of the neutrophil fraction of granulocytes from human blood by centrifugation on isotonic Nycodenz gradients.

3. Oligomeric coiled-coil adhesin YadA is a double-edged sword.

4. Effect of fluticasone propionate on neutrophil chemotaxis, superoxide generation, and extracellular proteolytic activity in vitro.

5. Methotrexate in neutrophils in children with acute lymphoblastic leukemia.

6. The effect of in vitro and in vivo dexamethasone on human neutrophil function.

7. Effect of ambroxol of neutrophil chemotaxis in vitro.

8. Methotrexate kinetics in myeloid bone marrow cells and peripheral neutrophils.

9. Effects of recombinant GM-CSF and IgA opsonisation on neutrophil phagocytosis of latex beads coated with P6 outer membrane protein from Haemophilus influenzae.

10. Superoxide production by Crohn's disease neutrophils.