Literature DB >> 3593982

A rapid method for the preparation of the neutrophil fraction of granulocytes from human blood by centrifugation on isotonic Nycodenz gradients.

T C Ford, R Needle, D Rickwood.   

Abstract

A rapid method for the preparation of neutrophils of the granulocyte fraction of human blood is described. A leucocyte-rich fraction is loaded onto preformed isotonic Nycodenz gradients, formed by mixing a stock isotonic Nycodenz solution with a NaCl diluent solution. After low speed centrifugation a neutrophil fraction of high viability, yield and purity can be isolated from the gradients.

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Year:  1987        PMID： 3593982     DOI： 10.1007/bf00626014

Source DB:  PubMed          Journal:  Blut        ISSN： 0006-5242


  12 in total

1.  Isolation of lymphocytes, granulocytes and macrophages.

Authors:  A Bøyum
Journal:  Scand J Immunol       Date:  1976-06       Impact factor: 3.487

2.  Isopycnic centrifugation of sheared chromatin in metrizamide gradients.

Authors:  D Rickwood; A Hell; G D Birnie
Journal:  FEBS Lett       Date:  1973-07-01       Impact factor: 4.124

3.  Ficoll-isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticulo-endothelial cells.

Authors:  J A Loos; D Roos
Journal:  Exp Cell Res       Date:  1974-06       Impact factor: 3.905

4.  Basophil leucocyte separation from human peripheral blood: a technique for their isolation in high purity and high yield.

Authors:  R P Day
Journal:  Clin Allergy       Date:  1972-09

5.  Eosinophil cell separation from human peripheral blood.

Authors:  R P Day
Journal:  Immunology       Date:  1970-06       Impact factor: 7.397

6.  Pharmacokinetics and biotransformation of iohexol in the rat and the dog.

Authors:  W Mützel; U Speck
Journal:  Acta Radiol Suppl       Date:  1980

7.  Isolation of human blood monocytes with Nycodenz, a new non-ionic iodinated gradient medium.

Authors:  A Bøyum
Journal:  Scand J Immunol       Date:  1983-05       Impact factor: 3.487

8.  Is Percoll innocuous to cells?

Authors:  J S Wakefield; J S Gale; M V Berridge; T W Jordan; H C Ford
Journal:  Biochem J       Date:  1982-03-15       Impact factor: 3.857

9.  A rapid one-step method for the isolation of human granulocytes from whole blood.

Authors:  L V Jepsen; T Skottun
Journal:  Scand J Clin Lab Invest       Date:  1982-05       Impact factor: 1.713

10.  Formation of isotonic Nycodenz gradients for cell separations.

Authors:  T C Ford; D Rickwood
Journal:  Anal Biochem       Date:  1982-08       Impact factor: 3.365
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  1 in total

1.  Dictyostelium amoebae and neutrophils can swim.

Authors:  Nicholas P Barry; Mark S Bretscher
Journal:  Proc Natl Acad Sci U S A       Date:  2010-06-08       Impact factor: 11.205
  1 in total

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