| Literature DB >> 7130620 |
Abstract
A simple procedure has been developed for purifying solubilized human liver glycoprotein sialyltransferase (EC 2.4.99.1) 16000-fold in 4-5% yield. The procedure involves two centrifugation steps, affinity chromatography of the ultrasupernatant fluid on cytidine diphosphate-hexanolamine-agarose followed by gel filtration on Sephadex G-150. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that the purified sialyltransferase preparation contains approximately equivalent amounts of three protein bands (with apparent molecular weights of 61000, 63000 and 70000) and is highly purified if not homogeneous.Entities:
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Year: 1982 PMID： 7130620 DOI： 10.1016/0165-022x(82)90045-8
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X