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Literature DB >> 3052425

Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography.

Abstract

We describe a simple three-step purification for Gal-beta 1,4-GlcNAc-alpha 2,6-sialyltransferase (EC 2.4.99.1) from rat liver which uses chromatography on Cibacron Blue F3GA and f.p.l.c. It gives a highly purified (11,000-fold) enzyme in 19% yield, which is free of other sialyltransferases, CMP-NeuAc hydrolase, sialidases and proteinases.

Entities: Chemical Species

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Year: 1988 PMID： 3052425 PMCID： PMC1149336 DOI： 10.1042/bj2530577

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.

Authors: M Potier; L Mameli; M Bélisle; L Dallaire; S B Melançon
Journal: Anal Biochem Date: 1979-04-15 Impact factor: 3.365

Review 2. The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins.

Authors: G Ashwell; A G Morell
Journal: Adv Enzymol Relat Areas Mol Biol Date: 1974

Review 3. Protein purification using immobilised triazine dyes.

Authors: P D Dean; D H Watson
Journal: J Chromatogr Date: 1979-10-01

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Simulation of water movement across the cell membrane in a hypertonic medium.

Authors: S K Devi; R Moore; N Davids; A F Findeis; R L Berger
Journal: Comput Biol Med Date: 1970-12 Impact factor: 4.589

Review 6. Comparative aspects of glycoprotein structure.

Authors: R Kornfeld; S Kornfeld
Journal: Annu Rev Biochem Date: 1976 Impact factor: 23.643

7. Purification of human liver glycoprotein sialyltransferase by affinity chromatography.

Authors: J A Alhadeff; R T Holzinger
Journal: J Biochem Biophys Methods Date: 1982-08

8. Purification to homogeneity and enzymatic characterization of an alpha-N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase from porcine submaxillary glands.

Authors: J E Sadler; J I Rearick; R L Hill
Journal: J Biol Chem Date: 1979-07-10 Impact factor: 5.157

Purification of alpha 2,6-sialyltransferase from rat liver by dye chromatography.

1. Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.

Review 2. The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins.

Review 3. Protein purification using immobilised triazine dyes.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Simulation of water movement across the cell membrane in a hypertonic medium.

Review 6. Comparative aspects of glycoprotein structure.

7. Purification of human liver glycoprotein sialyltransferase by affinity chromatography.

8. Purification to homogeneity and enzymatic characterization of an alpha-N-acetylgalactosaminide alpha 2 leads to 6 sialyltransferase from porcine submaxillary glands.

9. Activation and transfer of novel synthetic 9-substituted sialic acids.

10. Purification of a sialyltransferase from bovine colostrum by affinity chromatography on CDP-agarose.

1. Enzymatic transfer of sialic acids modified at C-5 employing four different sialyltransferases.

Review 2. The sialyl-alpha2,6-lactosaminyl-structure: biosynthesis and functional role.

3. Purification and characterization of alpha (2-6)-sialyltransferase from human liver.