Literature DB >> 7115882

Determination of fluorescence polarization of membrane probes in intact erythrocytes. Possible scattering artifacts.

H Kutchai, V H Huxley, L H Chandler.   

Abstract

The anisotropy of the fluorescence of diphenylhexatriene has been reported to be less in the membranes of intact erythrocytes than in erythrocyte ghost membranes or in membranes prepared from erythrocyte lipids. Evidence is presented that this may be an artifact due to the intense light scattering by the intact erythrocytes.

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Year:  1982        PMID： 7115882      PMCID： PMC1328936          DOI： 10.1016/S0006-3495(82)84512-8

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  10 in total

1.  CHROMATOGRAPHICALLY HOMOGENEOUS LECITHIN FROM EGG PHOSPHOLIPIDS.

Authors:  W S SINGLETON; M S GRAY; M L BROWN; J L WHITE
Journal:  J Am Oil Chem Soc       Date:  1965-01       Impact factor: 1.849

2.  Phosphorus assay in column chromatography.

Authors:  G R BARTLETT
Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

3.  Light-scattering effects in the measurement of membrane microviscosity with diphenylhexatriene.

Authors:  B R Lentz; B M Moore; D A Barrow
Journal:  Biophys J       Date:  1979-03       Impact factor: 4.033

4.  Microviscosity and order in the hydrocarbon region of micelles and membranes determined with fluorescent probes. I. Synthetic micelles.

Authors:  M Shinitzky; A C Dianoux; C Gitler; G Weber
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

5.  Dynamics of erythrocyte lipids in intact cells, in ghost membranes and in liposomes.

Authors:  B Aloni; M Shinitzky; A Livne
Journal:  Biochim Biophys Acta       Date:  1974-06-26

6.  Fluorescence depolarization by light-scattering in turbid solutions.

Authors:  F W Teale
Journal:  Photochem Photobiol       Date:  1969-12       Impact factor: 3.421

7.  Studies on phosphatidylcholine vesicles. Formation and physical characteristics.

Authors:  C Huang
Journal:  Biochemistry       Date:  1969-01       Impact factor: 3.162

8.  Monitoring volume changes in red cell ghosts by means of a trapped fluorescent probe.

Authors:  H Kutchai
Journal:  J Biochem Biophys Methods       Date:  1981-06

9.  Heterogeneity in the fluidity of intact erythrocyte membrane and its homogenization upon hemolysis.

Authors:  K I Tanaka; S Ohnishi
Journal:  Biochim Biophys Acta       Date:  1976-03-05

10.  Elevated microviscosity in membranes of erythrocytes affected by hereditary spherocytosis.

Authors:  B Aloni; M Shinitzky; S Moses; A Livne
Journal:  Br J Haematol       Date:  1975-09       Impact factor: 6.998
  10 in total
  4 in total

1.  The effect of erythrocyte associated light scattering on membrane fluorescence polarization.

Authors:  B F Dickens; T R Snow; V Green; W B Weglicki
Journal:  Mol Cell Biochem       Date:  1988-01       Impact factor: 3.396

2.  Fluorometry of turbid and absorbant samples and the membrane fluidity of intact erythrocytes.

Authors:  J Eisinger; J Flores
Journal:  Biophys J       Date:  1985-07       Impact factor: 4.033

3.  Water permeability through biological membranes by isotopic effects of fluorescence and light scattering.

Authors:  R Lawaczeck
Journal:  Biophys J       Date:  1984-03       Impact factor: 4.033

4.  The transverse organisation of ubiquinones in mitochondrial membranes as determined by fluorescence quenching. Evidence for a two-site model.

Authors:  R C Chatelier; W H Sawyer
Journal:  Eur Biophys J       Date:  1985       Impact factor: 1.733
  4 in total

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