Literature DB >> 262401

Light-scattering effects in the measurement of membrane microviscosity with diphenylhexatriene.

B R Lentz, B M Moore, D A Barrow.   

Abstract

Data from several membrane systems are presented to confirm an empirical means of correcting diphenylhexatriene fluorescence for depolarization caused by sample turbidity. The depolarization proportionally constants obtained are not equal, but are shown to vary with (a) the physical state of the membrane, (b) the cholesterol content of the membrane, (c) the protein content of the membrane, and (d) the method of membrane preparation or isolation. It is concluded that depolarization corrections must always be considered when diphenylhexatriene fluorescence anisotropy is used to compare the fluidities within different membrane bilayers.

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Year:  1979        PMID： 262401      PMCID： PMC1328486          DOI： 10.1016/S0006-3495(79)85318-7

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  13 in total

1.  Effects of sarcoplasmic reticulum Ca2+-ATPase on phospholipid bilayer fluidity: boundary lipid.

Authors:  B M Moore; B R Lentz; G Meissner
Journal:  Biochemistry       Date:  1978-11-28       Impact factor: 3.162

2.  Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 1. Single component phosphatidylcholine liposomes.

Authors:  B R Lentz; Y Barenholz; T E Thompson
Journal:  Biochemistry       Date:  1976-10-05       Impact factor: 3.162

3.  Interaction of retinol and intestinal microvillus membranes studied by flourescence polarization.

Authors:  D Schachter; U Cogan; M Shinitzky
Journal:  Biochim Biophys Acta       Date:  1976-11-02

4.  Microviscosity of the cell membrane.

Authors:  B Rudy; C Gitler
Journal:  Biochim Biophys Acta       Date:  1972-10-23

5.  Microviscosity and order in the hydrocarbon region of micelles and membranes determined with fluorescent probes. I. Synthetic micelles.

Authors:  M Shinitzky; A C Dianoux; C Gitler; G Weber
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

6.  Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate.

Authors:  M Shinitzky; Y Barenholz
Journal:  J Biol Chem       Date:  1974-04-25       Impact factor: 5.157

7.  Difference in microviscosity induced by different cholesterol levels in the surface membrane lipid layer of normal lymphocytes and malignant lymphoma cells.

Authors:  M Shinitzky; M Inbar
Journal:  J Mol Biol       Date:  1974-01-05       Impact factor: 5.469

8.  Factors influencing the lipid composition and fluidity of red cell membranes in vitro: production of red cells possessing more than two cholesterols per phospholipid.

Authors:  R A Cooper; M H Leslie; S Fischkoff; M Shinitzky; S J Shattil
Journal:  Biochemistry       Date:  1978-01-24       Impact factor: 3.162

9.  Dynamic structure of lipid bilayers studied by nanosecond fluorescence techniques.

Authors:  S Kawato; K Kinosita; A Ikegami
Journal:  Biochemistry       Date:  1977-05-31       Impact factor: 3.162

10.  Fluorescence and calorimetric studies of phase transitions in phosphatidylcholine multilayers: kinetics of the pretransition.

Authors:  B R Lentz; E Freire; R L Biltonen
Journal:  Biochemistry       Date:  1978-10-17       Impact factor: 3.162
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  26 in total

1.  Effect of capsaicin on ligand binding activity of the hippocampal serotonin1A receptor.

Authors:  Rajesh Prasad; Pushpendra Singh; Amitabha Chattopadhyay
Journal:  Glycoconj J       Date:  2008-09-19       Impact factor: 2.916

2.  Effect of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium acetobutylicum.

Authors:  S H Baer; H P Blaschek; T L Smith
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

3.  Cyclosporin A reduces canalicular membrane fluidity and regulates transporter function in rats.

Authors:  S Yasumiba; S Tazuma; H Ochi; K Chayama; G Kajiyama
Journal:  Biochem J       Date:  2001-03-15       Impact factor: 3.857

4.  Ultimate use of two-photon fluorescence microscopy to map orientational behavior of fluorophores.

Authors:  Patrick Ferrand; Paulina Gasecka; Alla Kress; Xiao Wang; Fatma-Zohra Bioud; Julien Duboisset; Sophie Brasselet
Journal:  Biophys J       Date:  2014-06-03       Impact factor: 4.033

5.  Modifying hepatic phospholipid synthesis associates with biliary phospholipid secretion rate in a transporter-independent manner in rats: relation to canalicular membrane fluidity.

Authors:  S Yasumiba; S Tazuma; H Ochi; G Kajiyama
Journal:  Dig Dis Sci       Date:  2001-06       Impact factor: 3.199

6.  Activation of the human red cell calcium ATPase by calcium pretreatment.

Authors:  J Fermin; P J Romero
Journal:  J Membr Biol       Date:  1994-02       Impact factor: 1.843

7.  Use of fluorescent probes that form intramolecular excimers to monitor structural changes in model and biological membranes.

Authors:  R L Melnick; H C Haspel; M Goldenberg; L M Greenbaum; S Weinstein
Journal:  Biophys J       Date:  1981-06       Impact factor: 4.033

8.  Asymmetry of lipid organization in cholinergic synaptic vesicle membranes.

Authors:  D M Michaelson; G Barkai; Y Barenholz
Journal:  Biochem J       Date:  1983-04-01       Impact factor: 3.857

9.  Simplified derivation of angular order and dynamics of rodlike fluorophores in models and membranes. Simultaneous estimation of the order and fluidity parameters for diphenylhexatriene by only coupling steady-state illumination polarization and lifetime of fluorescence.

Authors:  F Hare
Journal:  Biophys J       Date:  1983-06       Impact factor: 4.033

10.  The microviscosity of liver plasma membranes of rats fed with oleoylanilide.

Authors:  R Pagani; M T Portoles; F G Gavilanes; P Garcia-Barreno; A M Municio
Journal:  Biochem J       Date:  1984-02-15       Impact factor: 3.857
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