Literature DB >> 7106769

A simple reproducible method for prometaphase chromosome analysis.

J Rybak, A Tharapel, S Robinett, M Garcia, C Mankinen, M Freeman.   

Abstract

A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.

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Year:  1982        PMID： 7106769     DOI： 10.1007/BF00569213

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  36 in total

1.  Actinomycin D effects on mitosis and chromosomes: sticky chromatids and localized lesions.

Authors:  S Pathak; M McGill; T C Hsu
Journal:  Chromosoma       Date:  1975       Impact factor: 4.316

2.  Letter: Preferred base sites of actinomycin-deoxyribonucleic acid bindings by circular dichroism.

Authors:  F S Allen; R P Moen; U Hollstein
Journal:  J Am Chem Soc       Date:  1976-02-04       Impact factor: 15.419

3.  New technique for distinguishing between human chromosomes.

Authors:  A T Sumner; H J Evans; R A Buckland
Journal:  Nat New Biol       Date:  1971-07-07

4.  Analysis of the human karyotype using a reassociation technique.

Authors:  W Schnedl
Journal:  Chromosoma       Date:  1971       Impact factor: 4.316

5.  Chromosome structure and function in man. IV. Provisional maps of the three long acrocentric autosomes (chromosomes 13, 14, and 15) at pachytene in the male.

Authors:  D A Hungerford; G U LaBadie; G B Balaban; L R Messatzzia; G Haller; A E Miller
Journal:  Ann Genet       Date:  1971-12

6.  High-resolution bands in human fibroblast chromosomes induced by actinomycin D.

Authors:  R L Yu; M M Aronson; W W Nichols
Journal:  Cytogenet Cell Genet       Date:  1981

7.  Pachytene chromomere maps of human chromosomes 19 and 20.

Authors:  D A Hungerford; A M Hungerford
Journal:  Cytogenet Cell Genet       Date:  1980

8.  Leukocytes cultured from small inocula of whole blood and the preparation of metaphase chromosomes by treatment with hypotonic KCl.

Authors:  D A Hungerford
Journal:  Stain Technol       Date:  1965-11

9.  The C-group pachytene bivalent with a locus characteristic for parachromosomally situated particulate bodies (parameres): a provisional map in human males.

Authors:  D A Hungerford; F T Ashton; G B Balaban; G U LaBadie; L R Messatzzia; G Haller; A E Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  The characterization of high-resolution G-banded chromosomes of man.

Authors:  J J Yunis; J R Sawyer; D W Ball
Journal:  Chromosoma       Date:  1978-08-14       Impact factor: 4.316
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  14 in total

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Authors:  Usha Dave; Dhanlaxmi Shetty
Journal:  J Obstet Gynaecol India       Date:  2013-09-29

2.  Clinical and cytogenetic survey of 39 individuals with Prader-Labhart-Willi syndrome.

Authors:  M G Butler; F J Meaney; C G Palmer
Journal:  Am J Med Genet       Date:  1986-03

3.  Molecular structure and chromosomal mapping of the human homolog of the agouti gene.

Authors:  H Y Kwon; S J Bultman; C Löffler; W J Chen; P J Furdon; J G Powell; A L Usala; W Wilkison; I Hansmann; R P Woychik
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-11       Impact factor: 11.205

4.  Comparison of prometaphase chromosome techniques with emphasis on the role of colcemid.

Authors:  J E Wiley; L M Sargent; S L Inhorn; L F Meisner
Journal:  In Vitro       Date:  1984-12

5.  A complex three breakpoint translocation involving chromosomes 2, 4, and 9 identified by meiotic investigations of a human male ascertained for subfertility.

Authors:  N Saadallah; M Hulten
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

6.  Mapping of the ribophorin II (RPN II) gene to human chromosome 20q12-q13.1 by in-situ hybridization.

Authors:  C Löffler; V V Rao; I Hansmann
Journal:  Hum Genet       Date:  1991-06       Impact factor: 4.132

7.  Chromosomal and molecular evolution in Asiatic wild asses.

Authors:  O A Ryder; L G Chemnick
Journal:  Genetica       Date:  1990       Impact factor: 1.082

8.  Occupational hydrocarbon exposure among fathers of Prader-Willi syndrome patients with and without deletions of 15q.

Authors:  S B Cassidy; A J Gainey; M G Butler
Journal:  Am J Hum Genet       Date:  1989-06       Impact factor: 11.025

9.  Gene mapping of Usher syndrome type IIa: localization of the gene to a 2.1-cM segment on chromosome 1q41.

Authors:  W J Kimberling; M D Weston; C Möller; A van Aarem; C W Cremers; J Sumegi; P S Ing; C Connolly; A Martini; M Milani
Journal:  Am J Hum Genet       Date:  1995-01       Impact factor: 11.025

10.  The gene for bone morphogenetic protein 2A (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization.

Authors:  V V Rao; C Löffler; J M Wozney; I Hansmann
Journal:  Hum Genet       Date:  1992-11       Impact factor: 4.132
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