Literature DB >> 7103942

Purification of human C3b inactivator by monoclonal-antibody affinity chromatography.

L Hsiung, A N Barclay, M R Brandon, E Sim, R R Porter.   

Abstract

Monoclonal antibody has been obtained to the human complement control protein C3b inactivator after immunization of mice with the enzyme prepared by conventional methods. Antibody from ascitic fluid was purified and coupled to Sepharose-CL-4B to give a specific affinity column, which was used to isolate C3b inactivator from human serum in 70% yield. The product was characterized by size, chain structure, amino acid analysis and proteolytic activity.

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Year:  1982        PMID： 7103942      PMCID： PMC1158222          DOI： 10.1042/bj2030293

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

1.  The demonstration in human serum of "conglutinogen-activating factor" and its effect on the third component of complement.

Authors:  P J Lachmann; H J Müller-Eberhard
Journal:  J Immunol       Date:  1968-04       Impact factor: 5.422

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Isolation, characterization and N-terminal sequences of the CNBr-cleavage peptides from human complement Factor B. Localization of a free thiol group and a sequence defining the site cleaved by factor D.

Authors:  D L Christie; J Gagnon
Journal:  Biochem J       Date:  1982-03-01       Impact factor: 3.857

4.  Pattern of degradation of human complement fragment, C3b.

Authors:  E Sim; A B Wood; L M Hsiung; R B Sim
Journal:  FEBS Lett       Date:  1981-09-14       Impact factor: 4.124

Review 5.  The proteolytic activation systems of complement.

Authors:  K B Reid; R R Porter
Journal:  Annu Rev Biochem       Date:  1981       Impact factor: 23.643

6.  Three naturally-occurring inhibitors of components of complement in guinea pig and rabbit serum.

Authors:  N Tamura; R A Nelson
Journal:  J Immunol       Date:  1967-09       Impact factor: 5.422

7.  Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.

Authors:  G Köhler; C Milstein
Journal:  Eur J Immunol       Date:  1976-07       Impact factor: 5.532

8.  Amino acid sequence around the thiol and reactive acyl groups of human complement component C4.

Authors:  R D Campbell; J Gagnon; R R Porter
Journal:  Biochem J       Date:  1981-11-01       Impact factor: 3.857

9.  Human complement component C4. Structural studies on the fragments derived from C4b by cleavage with C3b inactivator.

Authors:  E M Press; J Gagnon
Journal:  Biochem J       Date:  1981-11-01       Impact factor: 3.857

10.  The catalytic chain of human complement subcomponent C1r. Purification and N-terminal amino acid sequences of the major cyanogen bromide-cleavage fragments.

Authors:  G J Arlaud; J Gagnon; R R Porter
Journal:  Biochem J       Date:  1982-01-01       Impact factor: 3.857
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  50 in total

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Journal:  Infect Immun       Date:  2001-06       Impact factor: 3.441

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Authors:  E Kenny; D Mason; A Pombo; F Ramírez
Journal:  Immunology       Date:  2000-10       Impact factor: 7.397

3.  Membrane-associated complement factor H on lymphoblastoid cell lines Raji expresses a co-factor activity for the factor I-mediated cleavage of C3b.

Authors:  M J Demares
Journal:  Immunology       Date:  1989-08       Impact factor: 7.397

4.  Multivalent recombinant proteins for probing functions of leucocyte surface proteins such as the CD200 receptor.

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5.  Experimental Acanthamoeba keratitis: II. Immunohistochemical evaluation.

Authors:  D F Larkin; D L Easty
Journal:  Br J Ophthalmol       Date:  1991-07       Impact factor: 4.638

6.  Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.

Authors:  W A Jefferies; M R Brandon; A F Williams; S V Hunt
Journal:  Immunology       Date:  1985-02       Impact factor: 7.397

7.  Population dynamics of lymphocyte subsets in the central nervous system of rats with different susceptibility to coronavirus-induced demyelinating encephalitis.

Authors:  R Dörries; S Schwender; H Imrich; H Harms
Journal:  Immunology       Date:  1991-11       Impact factor: 7.397

8.  The sensitivity of rat CD8+ and CD4+ T cells to ricin in vivo and in vitro and their relationship to IgE regulation.

Authors:  D Diaz-Sanchez; D M Kemeny
Journal:  Immunology       Date:  1990-01       Impact factor: 7.397

9.  Elimination of IgE regulatory rat CD8+ T cells in vivo differentially modulates interleukin-4 and interferon-gamma but not interleukin-2 production by splenic T cells.

Authors:  D Diaz-Sanchez; A Noble; D Z Staynov; T H Lee; D M Kemeny
Journal:  Immunology       Date:  1993-04       Impact factor: 7.397

10.  Effects of CD8 depletion on retinal soluble antigen induced experimental autoimmune uveoretinitis.

Authors:  V L Calder; Z S Zhao; Y Wang; K Barton; S L Lightman
Journal:  Immunology       Date:  1993-06       Impact factor: 7.397
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