Literature DB >> 7099964

Synthesis of mixed oligodeoxyribonucleotides following the solid phase method.

A Elmblad, S Josephson, G Palm.   

Abstract

A method for the synthesis of mixed dimers, trimers and oligonucleotides on a solid support using monomeric protected nucleoside phosphochloridites (1a-d) has been developed and the different nucleoside reagents, and the results show that yields of different oligomers in a mixture could be directly correlated to the concentration of the four reagents. Separation of mixed oligomers on a reversed phase C18 column has also been studied.

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Year:  1982        PMID： 7099964      PMCID： PMC320707          DOI： 10.1093/nar/10.10.3291

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Synthesis of thymidine oligonucleotides by phosphite triester intermediates.

Authors:  R L Letsinger; W B Lunsford
Journal:  J Am Chem Soc       Date:  1976-06-09       Impact factor: 15.419

2.  CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40.

Authors:  H Weber; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

3.  Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates.

Authors:  G W Daub; E E van Tamelen
Journal:  J Am Chem Soc       Date:  1977-05-11       Impact factor: 15.419

4.  Synthesis of oligodeoxyribonucleotides on silica gel support.

Authors:  F Chow; T Kempe; G Palm
Journal:  Nucleic Acids Res       Date:  1981-06-25       Impact factor: 16.971

5.  Sequence analysis of short DNA fragments.

Authors:  C P Tu; R Wu
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Chemical synthesis of deoxyoligonucleotides by the modified triester method.

Authors:  S A Narang; R Brousseau; H M Hsiung; J J Michniewicz
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

7.  New chemical methods for synthesizing polynucleotides.

Authors:  M H Caruthers; S L Beaucage; J W Efcavitch; E F Fisher; M D Matteucci; Y Stabinsky
Journal:  Nucleic Acids Symp Ser       Date:  1980

8.  Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

Authors:  M J Gait; M Singh; R C Sheppard; M D Edge; A R Greene; G R Heathcliffe; T C Atkinson; C R Newton; A F Markham
Journal:  Nucleic Acids Res       Date:  1980-03-11       Impact factor: 16.971

9.  The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.

Authors:  R B Wallace; M J Johnson; T Hirose; T Miyake; E H Kawashima; K Itakura
Journal:  Nucleic Acids Res       Date:  1981-02-25       Impact factor: 16.971
  9 in total
  10 in total

1.  Label-free optical detection of peptide synthesis on a porous silicon scaffold/sensor.

Authors:  Patrick Furbert; Caiyan Lu; Nicholas Winograd; Lisa DeLouise
Journal:  Langmuir       Date:  2008-02-05       Impact factor: 3.882

2.  Molecular cloning of the temperature-inducible outer membrane protein 1 of Yersinia pseudotuberculosis.

Authors:  I Bölin; H Wolf-Watz
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441

3.  An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.

Authors:  B Nilsson; M Uhlén; S Josephson; S Gatenbeck; L Philipson
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

4.  Isolation of cDNA sequences coding for a part of human tissue plasminogen activator.

Authors:  T Edlund; T Ny; M Rånby; L O Hedén; G Palm; E Holmgren; S Josephson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

5.  Structure of soybean Kunitz trypsin inhibitor mRNA determined from cDNA by using oligodeoxynucleotide primers.

Authors:  L M Hoffman; C Sengupta-Gopalan; H E Paaren
Journal:  Plant Mol Biol       Date:  1984-03       Impact factor: 4.076

6.  Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II.

Authors:  B Hammarberg; P A Nygren; E Holmgren; A Elmblad; M Tally; U Hellman; T Moks; M Uhlén
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

7.  Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method.

Authors:  Y Ike; S Ikuta; M Sato; T Huang; K Itakura
Journal:  Nucleic Acids Res       Date:  1983-01-25       Impact factor: 16.971

8.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

9.  Analytical studies of 'mixed sequence' oligodeoxyribonucleotides synthesized by competitive coupling of either methyl- or beta-cyanoethyl-N,N-diisopropylamino phosphoramidite reagents, including 2'-deoxyinosine.

Authors:  G Zon; K A Gallo; C J Samson; K L Shao; M F Summers; R A Byrd
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971

10.  Production of specific antibodies against protein A fusion proteins.

Authors:  B Löwenadler; B Nilsson; L Abrahmsén; T Moks; L Ljungqvist; E Holmgren; S Paleus; S Josephson; L Philipson; M Uhlén
Journal:  EMBO J       Date:  1986-09       Impact factor: 11.598
  10 in total

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