Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Nonenzymatic methylation of DNA by S-adenosylmethionine in vitro.

Literature DB >> 7083475

Nonenzymatic methylation of DNA by S-adenosylmethionine in vitro.

Abstract

S-Adenosylmethionine was found to methylate DNA non-enzymatically to produce the same putative promutagenic and procarcinogenic lesions formed by carcinogenic chemical methylating agents. The formation of 7-methylguanine was confirmed by u.v. spectrophotometry, the formation of O6-methylguanine and 3-methyladenine was suggested by the cochromatography of radioactivity with standard bases. It is possible that this reaction may explain the presence of constitutive cellular enzymes specifically for the repair of methylated DNA, and may indicate the mechanism whereby methylated guanine is formed in the liver DNA of rats with chemically induced liver damage.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1982 PMID： 7083475 DOI： 10.1093/carcin/3.3.349

Source DB: PubMed Journal: Carcinogenesis ISSN： 0143-3334 Impact factor: 4.944

Keyword Cloud
Cited

50 in total

1. Lack of the DNA repair protein O6-methylguanine-DNA methyltransferase in histologically normal brain adjacent to primary human brain tumors.

Authors: J R Silber; A Blank; M S Bobola; B A Mueller; D D Kolstoe; G A Ojemann; M S Berger
Journal: Proc Natl Acad Sci U S A Date: 1996-07-09 Impact factor: 11.205

2. The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions.

Authors: W Xiao; L Samson
Journal: Nucleic Acids Res Date: 1992-07-25 Impact factor: 16.971

Review 3. Direct reversal of DNA alkylation damage.

Authors: Yukiko Mishina; Erica M Duguid; Chuan He
Journal: Chem Rev Date: 2006-02 Impact factor: 60.622

Review 4. Oxidative dealkylation DNA repair mediated by the mononuclear non-heme iron AlkB proteins.

Authors: Yukiko Mishina; Chuan He
Journal: J Inorg Biochem Date: 2006-02-15 Impact factor: 4.155

5. Site-specific synthesis and characterization of oligonucleotides containing an N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion, the ring-opened product from N7-methylation of deoxyguanosine.

Authors: Plamen P Christov; Kyle L Brown; Ivan D Kozekov; Michael P Stone; Thomas M Harris; Carmelo J Rizzo
Journal: Chem Res Toxicol Date: 2008-12 Impact factor: 3.739

Nonenzymatic methylation of DNA by S-adenosylmethionine in vitro.

1. Lack of the DNA repair protein O6-methylguanine-DNA methyltransferase in histologically normal brain adjacent to primary human brain tumors.

2. The Saccharomyces cerevisiae MGT1 DNA repair methyltransferase gene: its promoter and entire coding sequence, regulation and in vivo biological functions.

Review 3. Direct reversal of DNA alkylation damage.

Review 4. Oxidative dealkylation DNA repair mediated by the mononuclear non-heme iron AlkB proteins.

5. Site-specific synthesis and characterization of oligonucleotides containing an N6-(2-deoxy-D-erythro-pentofuranosyl)-2,6-diamino-3,4-dihydro-4-oxo-5-N-methylformamidopyrimidine lesion, the ring-opened product from N7-methylation of deoxyguanosine.

6. Mutagenicity of N3-methyladenine: a multi-translesion polymerase affair.

7. In vivo evidence for endogenous DNA alkylation damage as a source of spontaneous mutation in eukaryotic cells.

Review 8. Tissue aging: the integration of collective and variant responses of cells to entropic forces over time.

9. DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.

10. Effect of n3-methyladenine and an isosteric stable analogue on DNA polymerization.