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Literature DB >> 7068817

Production and characterization of a hybridoma-derived antibody to Blastomyces dermatitidis.

Abstract

A hybridoma cell line was isolated which produced monoclonal antibody to one protein component of a yeast-phase cytoplasmic antigenic complex of Blastomyces dermatitidis. The immunoglobulin M antibody product was characterized by immunodiffusion, autoradiography of polyacrylamide gels, and cellulose acetate electrophoresis. By attaching the antibody to an affinity gel, one major protein band was identified by polyacrylamide gel electrophoresis as the antigen for which the antibody was specific.

Entities: Chemical Species

Mesh：

Substances：
Antibodies, Fungal

Year: 1982 PMID： 7068817 PMCID： PMC272060 DOI： 10.1128/jcm.15.2.204-207.1982

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

2. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors: R A Laskey; A D Mills
Journal: Eur J Biochem Date: 1975-08-15

3. A miniaturized system for electrophoresis on polyacrylamide gels.

Authors: Z I Ogita; C L Markert
Journal: Anal Biochem Date: 1979-11-01 Impact factor: 3.365

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

5. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells.

Authors: R H Kennett; K A Denis; A S Tung; N R Klinman
Journal: Curr Top Microbiol Immunol Date: 1978 Impact factor: 4.291

6. A better cell line for making hybridomas secreting specific antibodies.

Authors: M Shulman; C D Wilde; G Köhler
Journal: Nature Date: 1978-11-16 Impact factor: 49.962

7. Yeast- and mycelial-phase antigens of Blastomyces dermatitidis: comparison using disc gel electrophoresis.

Authors: R A Cox; H W Larsh
Journal: Infect Immun Date: 1974-07 Impact factor: 3.441

8. Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis.

Authors: R A Cox; H W Larsh
Journal: Infect Immun Date: 1974-07 Impact factor: 3.441

9. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.

Authors: M L Gefter; D H Margulies; M D Scharff
Journal: Somatic Cell Genet Date: 1977-03

10. Cross-reactivity between antigens of Coccidioides immitis, Histoplasma capsulatum and Blastomyces dermatitidis in lymphocyte transformation assays.

Authors: R A Cox
Journal: Infect Immun Date: 1979-09 Impact factor: 3.441

6 in total

Production and characterization of a hybridoma-derived antibody to Blastomyces dermatitidis.

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

2. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

3. A miniaturized system for electrophoresis on polyacrylamide gels.

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

5. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells.

6. A better cell line for making hybridomas secreting specific antibodies.

7. Yeast- and mycelial-phase antigens of Blastomyces dermatitidis: comparison using disc gel electrophoresis.

8. Isolation of skin test-active preparations from yeast-phase cells of Blastomyces dermatitidis.

9. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.

10. Cross-reactivity between antigens of Coccidioides immitis, Histoplasma capsulatum and Blastomyces dermatitidis in lymphocyte transformation assays.

1. Production and characterization of monoclonal antibodies to cell wall antigens of Aspergillus fumigatus.

2. Production and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens.

Review 3. Antigens and chemical composition of Blastomyces dermatitidis.

4. Production and characterization of three monoclonal antibodies to Candida albicans proteins.

5. Production and characterization of yeast killer toxin monoclonal antibodies.

6. Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis.