Literature DB >> 605383

A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells.

M L Gefter, D H Margulies, M D Scharff.   

Abstract

A simple method is described for promoting the fusion of mouse myeloma cells in suspension with polyethylene glycol (PEG 1000). By carefully controlling the concentraion of PEG and the time of exposure of the cells, it was possible to obtain hybridization frequencies several-hundred-fold higher than those obtained with Sendai virus.

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Year:  1977        PMID： 605383     DOI： 10.1007/bf01551818

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  147 in total

1.  B-cell proliferation initiated by Ia cross-linking and sustained by interleukins leads to class switching but not somatic mutation in vitro.

Authors:  L J Wysocki; G Creadon; K R Lehmann; J C Cambier
Journal:  Immunology       Date:  1992-01       Impact factor: 7.397

2.  Transcriptional down-regulation of N-myc expression during B-cell development.

Authors:  R K Smith; K Zimmerman; G D Yancopoulos; A Ma; F W Alt
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

3.  Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.

Authors:  R A Deich; B J Metcalf; C W Finn; J E Farley; B A Green
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

4.  Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies.

Authors:  H Tiedge; H Lünsdorf; G Schäfer; H U Schairer
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

5.  Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera.

Authors:  R A Deich; A Anilionis; J Fulginiti; B J Metcalf; S Quataert; T Quinn-Dey; G W Zlotnick; B A Green
Journal:  Infect Immun       Date:  1990-10       Impact factor: 3.441

6.  Production and characterization of a monoclonal antibody to the complement fixation antigen of Coccidioides immitis.

Authors:  M J Dolan; R A Cox
Journal:  Infect Immun       Date:  1991-06       Impact factor: 3.441

7.  Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.

Authors:  S Fachon-Kalweit; B L Elder; P Fives-Taylor
Journal:  Infect Immun       Date:  1985-06       Impact factor: 3.441

8.  Membrane 126-kilodalton phosphoglycoprotein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells.

Authors:  H R Soule; E Linder; T S Edgington
Journal:  Proc Natl Acad Sci U S A       Date:  1983-03       Impact factor: 11.205

9.  Identification and preliminary characterization of a Streptococcus sanguis fibrillar glycoprotein.

Authors:  E J Morris; N Ganeshkumar; M Song; B C McBride
Journal:  J Bacteriol       Date:  1987-01       Impact factor: 3.490

10.  Immunopathologic aspects of woodchuck hepatitis.

Authors:  D Frommel; D Crevat; L Vitvitsky; C Pichoud; O Hantz; M Chevalier; J A Grimaud; J Lindberg; C G Trépo
Journal:  Am J Pathol       Date:  1984-04       Impact factor: 4.307
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