Literature DB >> 7061870

Use of an enzyme-linked immunosorbent assay for screening hybridoma antibodies against hepatitis B surface antigen.

A Boniolo, M Dovis, R Matteja.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for screening production of monoclonal antibodies with specificity for HBsAg. Mouse hybridoma IgG were firstly extracted from assay medium with goat anti-mouse IgG adsorbed on polystyrene beads. The specific antibody was revealed by saturation with HBs antigen from human positive sera followed by reaction with specific sheep anti-HBs antibody conjugated with peroxidase. The sensitivity was of the order of 0.5-1 ng/ml specific antibody and specificity was satisfactory. The assay permits easy identification of determinant specificity on screening.

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Year:  1982        PMID： 7061870     DOI： 10.1016/0022-1759(82)90361-1

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

1.  Clinical benefits of biochemical markers of bone turnover in Egyptian children with chronic liver diseases.

Authors:  Karam A Mahdy; Hanaa H Ahmed; Fathia Mannaa; Azza Abdel-Shaheed
Journal:  World J Gastroenterol       Date:  2007-02-07       Impact factor: 5.742

2.  Relationship between the intrahepatic expression of 'e' and 'c' epitopes of the nucleocapsid protein of hepatitis B virus and viraemia.

Authors:  M Ballaré; C Lavarini; M R Brunetto; E Petruzzelli; M Dovis; G Molino; F Bonino
Journal:  Clin Exp Immunol       Date:  1989-01       Impact factor: 4.330

3.  A simple and sensitive solid-phase radioimmunoassay for the assay of human TSH antibody. Use in screening hybridoma cell lines.

Authors:  S Iwasa; S Pincus; E Saito; N P Laborde; W D Odell
Journal:  Appl Biochem Biotechnol       Date:  1983-08       Impact factor: 2.926

4.  Production of monoclonal antibodies to hepatitis B surface and core antigens, and use in the detection of viral antigens in liver biopsies.

Authors:  R S Tedder; P Guarascio; J L Yao; R B Lord; A L Eddleston
Journal:  J Hyg (Lond)       Date:  1983-02

5.  Endobronchial administration of iodine-131 B72.3 monoclonal antibody in patients with lung cancer.

Authors:  S Del Vecchio; L Mansi; A Petrillo; L Camera; M Sofia; A Marra; L Carratù; M Salvatore
Journal:  Eur J Nucl Med       Date:  1991

6.  European multicentre study on melanoma immunoscintigraphy by means of 99mTc-labelled monoclonal antibody fragments. The European Multicentre Study Group.

Authors:  A G Siccardi; G L Buraggi; P G Natali; G A Scassellati; G Viale; S Ferrone
Journal:  Eur J Nucl Med       Date:  1990

7.  Kinetics, quantitative analysis and radioimmunolocalization using indium-111-HMFG1 monoclonal antibody in patients with breast cancer.

Authors:  H P Kalofonos; J M Sackier; M Hatzistylianou; S Pervez; J Taylor-Papadimitriou; J H Waxman; J P Lavender; C Wood; A A Epenetos
Journal:  Br J Cancer       Date:  1989-06       Impact factor: 7.640
  7 in total

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