Literature DB >> 7048302

Immunocytochemical localization of tubulin, actin, and myosin in axonemes of ciliated cells from quail oviduct.

D Sandoz, P Gounon, E Karsenti, M E Sauron.   

Abstract

Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes are poorly stained. Actin antibodies clearly stain the axonemes, but only on the proximal-half portion, whereas myosin antibodies stain a small area of the axonemes just above the ciliary neck region.

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Year:  1982        PMID: 7048302      PMCID: PMC346382          DOI: 10.1073/pnas.79.10.3198

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  17 in total

1.  Studies on reactivated cilia. II. Reactivation of ciliated cortices from the oviduct of Anolis cristatellus.

Authors:  L D Torres; F L Renaud; C Portocarrero
Journal:  Exp Cell Res       Date:  1977-09       Impact factor: 3.905

2.  A protein factor essential for microtubule assembly.

Authors:  M D Weingarten; A H Lockwood; S Y Hwo; M W Kirschner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-05       Impact factor: 11.205

3.  Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii.

Authors:  G Piperno; B Huang; D J Luck
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

4.  Detection of tubulin and actin in various cell lines by an immunoperoxidase technique.

Authors:  E Karsenti; B Guilbert; M Bornens; S Avrameas; R Whalen; D Pantaloni
Journal:  J Histochem Cytochem       Date:  1978-11       Impact factor: 2.479

5.  Microtubule assembly in the absence of added nucleotides.

Authors:  M L Shelanski; F Gaskin; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

6.  Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.

Authors:  S Avrameas; T Ternynck
Journal:  Immunochemistry       Date:  1971-12

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors:  R C Graham; M J Karnovsky
Journal:  J Histochem Cytochem       Date:  1966-04       Impact factor: 2.479

9.  Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. I. Antibodies to smooth and striated chicken muscle myosins.

Authors:  U Gröschel-Stewart; J Schreiber; C Mahlmeister
Journal:  Histochemistry       Date:  1976-02-26

10.  Isolation of ciliated or unciliated basal bodies from the rabbit oviduct.

Authors:  R G Anderson
Journal:  J Cell Biol       Date:  1974-02       Impact factor: 10.539

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  6 in total

1.  Tubulin evolution: ciliate-specific epitopes are conserved in the ciliary tubulin of Metazoa.

Authors:  A Adoutte; M Claisse; R Maunoury; J Beisson
Journal:  J Mol Evol       Date:  1985       Impact factor: 2.395

2.  Cytochalasin D inhibits basal body migration and ciliary elongation in quail oviduct epithelium.

Authors:  E Boisvieux-Ulrich; M C Lainé; D Sandoz
Journal:  Cell Tissue Res       Date:  1990-03       Impact factor: 5.249

3.  Electron microscopic immunochemical localization of actin in fibroblasts in healing skin and palate wounds of beagle dog.

Authors:  C A Squier; C S Leranth; S Ghoneim; C R Kremenak
Journal:  Histochemistry       Date:  1983

4.  Distinct cytoskeletal domains revealed in sperm cells.

Authors:  I Virtanen; R A Badley; R Paasivuo; V P Lehto
Journal:  J Cell Biol       Date:  1984-09       Impact factor: 10.539

5.  Myosin at the apical pole of ciliated epithelial cells as revealed by a monoclonal antibody.

Authors:  C Klotz; N Bordes; M C Laine; D Sandoz; M Bornens
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539

6.  The fate of the primary cilium during myofibroblast transition.

Authors:  Matthew Rozycki; Monika Lodyga; Jessica Lam; Maria Zena Miranda; Károly Fátyol; Pam Speight; András Kapus
Journal:  Mol Biol Cell       Date:  2014-01-08       Impact factor: 4.138

  6 in total

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