Literature DB >> 266200

Two-dimensional analysis of flagellar proteins from wild-type and paralyzed mutants of Chlamydomonas reinhardtii.

G Piperno, B Huang, D J Luck.   

Abstract

Flagellar polypeptides of Chlamydomonas reinhardtii were analyzed in two-dimensions by isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate. In addition to flagellar tubulin, over 130 polypeptides were resolved and 100 of these were identified as axonemal components in wild-type organisms. Flagella of two nonconditional paralyzed mutants, pf 14 and pf 1, were also analyzed and, at the same time, electron microscopic studies were carried out. pf 14 flagella, which completely lack radial spokes and associated spokeheads, are missing 12 polypeptides. Six of these polypeptides are also missing from pf 1 flagella in which spokes are clearly present but spoke heads appear to be absent.

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Year:  1977        PMID： 266200      PMCID： PMC430838          DOI： 10.1073/pnas.74.4.1600

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  Mutants of Chlamydomonas moewusii with impaired motility.

Authors:  R A LEWIN
Journal:  J Gen Microbiol       Date:  1954-12

2.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

3.  The three-dimensional arrangement of radial spokes in the flagella of Chlamydomonas reinhardii.

Authors:  D Chasey
Journal:  Exp Cell Res       Date:  1974-03-15       Impact factor: 3.905

4.  The structural basis of ciliary bend formation. Radial spoke positional changes accompanying microtubule sliding.

Authors:  F D Warner; P Satir
Journal:  J Cell Biol       Date:  1974-10       Impact factor: 10.539

Review 5.  The genetics of photosynthesis and of the chloroplast in Chlamydomonas reinhardi.

Authors:  R P Levine; U W Goodenough
Journal:  Annu Rev Genet       Date:  1970       Impact factor: 16.830

6.  Microtubular proteins of Chlamydomonas reinhardtii. An immunochemical study based on the use of an antibody specific for the beta-tubulin subunit.

Authors:  G Piperno; D J Luck
Journal:  J Biol Chem       Date:  1977-01-10       Impact factor: 5.157

7.  Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas.

Authors:  D L Ringo
Journal:  J Cell Biol       Date:  1967-06       Impact factor: 10.539

8.  Microtubules: evidence for 13 protofilaments.

Authors:  L G Tilney; J Bryan; D J Bush; K Fujiwara; M S Mooseker; D B Murphy; D H Snyder
Journal:  J Cell Biol       Date:  1973-11       Impact factor: 10.539

9.  Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii.

Authors:  B Huang; M R Rifkin; D J Luck
Journal:  J Cell Biol       Date:  1977-01       Impact factor: 10.539

10.  Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.

Authors:  G B Witman; K Carlson; J Berliner; J L Rosenbaum
Journal:  J Cell Biol       Date:  1972-09       Impact factor: 10.539
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  87 in total

Review 1.  Genetics and pulmonary medicine. 6. Immotile cilia syndrome: past, present, and prospects for the future.

Authors:  B A Afzelius
Journal:  Thorax       Date:  1998-10       Impact factor: 9.139

2.  Asymmetry of the central apparatus defines the location of active microtubule sliding in Chlamydomonas flagella.

Authors:  Matthew J Wargo; Elizabeth F Smith
Journal:  Proc Natl Acad Sci U S A       Date:  2002-12-23       Impact factor: 11.205

3.  Regulation of flagellar dynein by calcium and a role for an axonemal calmodulin and calmodulin-dependent kinase.

Authors:  Elizabeth F Smith
Journal:  Mol Biol Cell       Date:  2002-09       Impact factor: 4.138

4.  Two types of genetic interaction implicate the whirligig gene of Drosophila melanogaster in microtubule organization in the flagellar axoneme.

Authors:  L L Green; N Wolf; K L McDonald; M T Fuller
Journal:  Genetics       Date:  1990-12       Impact factor: 4.562

5.  The Rib43a protein is associated with forming the specialized protofilament ribbons of flagellar microtubules in Chlamydomonas.

Authors:  J M Norrander; A M deCathelineau; J A Brown; M E Porter; R W Linck
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

6.  Loss-of-function mutations in RSPH1 cause primary ciliary dyskinesia with central-complex and radial-spoke defects.

Authors:  Esther Kott; Marie Legendre; Bruno Copin; Jean-François Papon; Florence Dastot-Le Moal; Guy Montantin; Philippe Duquesnoy; William Piterboth; Daniel Amram; Laurence Bassinet; Julie Beucher; Nicole Beydon; Eric Deneuville; Véronique Houdouin; Hubert Journel; Jocelyne Just; Nadia Nathan; Aline Tamalet; Nathalie Collot; Ludovic Jeanson; Morgane Le Gouez; Benoit Vallette; Anne-Marie Vojtek; Ralph Epaud; André Coste; Annick Clement; Bruno Housset; Bruno Louis; Estelle Escudier; Serge Amselem
Journal:  Am J Hum Genet       Date:  2013-08-29       Impact factor: 11.025

7.  Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella.

Authors:  Chun Yang; Mark M Compton; Pinfen Yang
Journal:  Mol Biol Cell       Date:  2004-11-24       Impact factor: 4.138

8.  In vivo analyses of radial spoke transport, assembly, repair and maintenance.

Authors:  Karl F Lechtreck; Ilaria Mengoni; Batare Okivie; Kiersten B Hilderhoff
Journal:  Cytoskeleton (Hoboken)       Date:  2018-09-10

9.  Tubulin biosynthesis in the developmental cycle of a parasitic protozoan, Leishmania mexicana: changes during differentiation of motile and nonmotile stages.

Authors:  D Fong; K P Chang
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

Review 10.  Motility and more: the flagellum of Trypanosoma brucei.

Authors:  Gerasimos Langousis; Kent L Hill
Journal:  Nat Rev Microbiol       Date:  2014-07       Impact factor: 60.633
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