| Literature DB >> 7047570 |
E S Bos, A A van der Doelen, N van Rooy, A H Schuurs.
Abstract
The use of 3,3',5,5' - tetramethylbenzidine as non-mutagenic chromogen for the end point determination in enzyme-immunoassay (EIA) is described. In sandwich EIAs for HCG and HBsAg and in a competitive EIA for testosterone, the colour yield with TMB was superior to that obtained with o-phenylene diamine (OPD), which was by far the best chromogen for horse-radish peroxidase until now. This led to an improvement of sensitivity and precision of the assays and makes EIA even more competitive with other types of immunoassays.Entities:
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Year: 1981 PMID： 7047570 DOI： 10.1080/15321818108056977
Source DB: PubMed Journal: J Immunoassay ISSN： 0197-1522