Literature DB >> 24310656

The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco.

J F Antoniw1, R F White, D J Barbara, P Jones, A Longley.   

Abstract

An antiserum was prepared to the b1 protein purified from TMV infectedN. tabacum cv. Xanthi-nc leaves and used to study PR proteins. The Xanthi-nc proteins b2 and b3 were shown to be serologically closely related to b1. Antisera to b1 protein and TMV were used in a F(ab')2 enzyme linked immunosorbent assay to monitor PR protein and TMV concentrations, respectively, during the first 6 days of a systemic TMV infection (cv. Xanthi) and a localised TMV infection (cv. Xanthi-nc).

Entities:  

Year:  1985        PMID： 24310656     DOI： 10.1007/BF02498715

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  4 in total

1.  A simple indirect ELISA using F(ab')2 fragments of immunoglobulin.

Authors:  D J Barbara; M F Clark
Journal:  J Gen Virol       Date:  1982-02       Impact factor: 3.891

2.  Antigen controlled immuno diagnosis-- 'ACID test'.

Authors:  G Müller; C L Baigent
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

3.  [Hypersensitivity to viruses, temperature and soluble proteins in Nicotiana Xanthi n.c. Appearance of new macromolecules at the repression of viral synthesis].

Authors:  S Gianinazzi; C Martin; J C Vallée
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1970-05-11

4.  3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay.

Authors:  E S Bos; A A van der Doelen; N van Rooy; A H Schuurs
Journal:  J Immunoassay       Date:  1981
  4 in total
  7 in total

1.  Biochemical and serological characterization of b-proteins fromNicotiana species.

Authors:  P Ahl; J F Antoniw; R F White; S Gianinazzi
Journal:  Plant Mol Biol       Date:  1985-01       Impact factor: 4.076

2.  Identification, purification, and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves.

Authors:  L C van Loon; Y A Gerritsen; C E Ritter
Journal:  Plant Mol Biol       Date:  1987-11       Impact factor: 4.076

3.  Molecular analysis of two PR-1 pseudogenes from tobacco.

Authors:  A J Pfitzner; A Beilmann; H M Goodman; U M Pfitzner
Journal:  Plant Mol Biol       Date:  1991-01       Impact factor: 4.076

4.  Biological activities of human interferon and 2'-5' oligoadenylates in plants.

Authors:  O N Kulaeva; A B Fedina; E A Burkhanova; N N Karavaiko; I B Kaplan; M E Taliansky; J G Atabekov
Journal:  Plant Mol Biol       Date:  1992-11       Impact factor: 4.076

5.  Changes with time in the distribution of virus and PR protein around single local lesions of TMV infected tobacco.

Authors:  J F Antoniw; R F White
Journal:  Plant Mol Biol       Date:  1986-05       Impact factor: 4.076

6.  Purification and characterization of 8 of the pathogenesis-related proteins in tobacco leaves reacting hypersensitively to tobacco mosaic virus.

Authors:  E Jamet; B Fritig
Journal:  Plant Mol Biol       Date:  1986-03       Impact factor: 4.076

7.  Homology between chitinases that are induced by TMV infection of tobacco.

Authors:  R A Hooft van Huijsduijnen; S Kauffmann; F T Brederode; B J Cornelissen; M Legrand; B Fritig; J F Bol
Journal:  Plant Mol Biol       Date:  1987-07       Impact factor: 4.076
  7 in total

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