Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification and biological characterization of shiga toxin from Shigella dysenteriae 1.

Literature DB >> 7047403

Purification and biological characterization of shiga toxin from Shigella dysenteriae 1.

J E Brown, D E Griffin, S W Rothman, B P Doctor.

Abstract

Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and preparative isoelectric focusing in sucrose gradients. The purified toxin was resolved by discontinuous polyacrylamide gel electrophoresis into a major cytotoxic protein band and a closely migrating, cytotoxic protease-nicked minor band. Antiserum generated by immunization with glutaraldehyde-inactivated toxin was shown to be monospecific against S. dysenteriae cell lysates. This highly purified toxin was cytotoxic to HeLa cells, enterotoxic in rabbit ileal loops, and lethal to mice. Monospecific antiserum to the toxin neutralized completely these toxin activities in both purified toxin preparations and crude shigella cell lysates.

Entities: Chemical Species

Mesh：

Substances：

Year: 1982 PMID： 7047403 PMCID： PMC551430 DOI： 10.1128/iai.36.3.996-1005.1982

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

22 in total

1. The neurotoxin of Shigella shigae. I. Production, purification and properties of the toxin.

Authors: W E VAN HEYNINGEN; G P GLADSTONE
Journal: Br J Exp Pathol Date: 1953-04

2. Shigellosis due to Shigella dysenteriae. 1. Relative importance of mucosal invasion versus toxin production in pathogenesis.

Authors: P Gemski; A Takeuchi; O Washington; S B Formal
Journal: J Infect Dis Date: 1972-11 Impact factor: 5.226

3. Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin.

Authors: J E Brown; S W Rothman; B P Doctor
Journal: Infect Immun Date: 1980-07 Impact factor: 3.441

4. Production and characterization of exotoxin(s) of Shigella dysenteriae type 1.

Authors: J McIver; G F Grady; G T Keusch
Journal: J Infect Dis Date: 1975-05 Impact factor: 5.226

5. Inhibition of in vitro protein synthesis by Shigella dysenteriae 1 toxin.

Authors: M R Thompson; M S Steinberg; P Gemski; S B Formal; B P Doctor
Journal: Biochem Biophys Res Commun Date: 1976-08-09 Impact factor: 3.575

6. Shigella toxin inhibition of binding and translation of polyuridylic acid by Escherichia coli ribosomes.

Authors: J G Olenick; A D Wolfe
Journal: J Bacteriol Date: 1980-03 Impact factor: 3.490

7. Inhibition of small-intestinal sugar and amino acid transport by the enterotoxin of Shigella dysenteriae I.

Authors: H J Binder; D S Whiting
Journal: Infect Immun Date: 1977-05 Impact factor: 3.441

8. Isolation and characterization of Shigella shigae cytotoxin.

Authors: S Olsnes; K Eiklid
Journal: J Biol Chem Date: 1980-01-10 Impact factor: 5.157

9. The pathogenesis of Shigella diarrhea. I. Enterotoxin production by Shigella dysenteriae I.

Authors: G T Keusch; G F Grady; L J Mata; J McIver
Journal: J Clin Invest Date: 1972-05 Impact factor: 14.808

10. The cytotoxic activity of Shigella toxin. Evidence for catalytic inactivation of the 60 S ribosomal subunit.

Authors: R Reisbig; S Olsnes; K Eiklid
Journal: J Biol Chem Date: 1981-08-25 Impact factor: 5.157

31 in total

1. Purification of Shiga toxin and Shiga-like toxins I and II by receptor analog affinity chromatography with immobilized P1 glycoprotein and production of cross-reactive monoclonal antibodies.

Authors: A Donohue-Rolfe; D W Acheson; A V Kane; G T Keusch
Journal: Infect Immun Date: 1989-12 Impact factor: 3.441

Review 2. Shiga and Shiga-like toxins.

Authors: A D O'Brien; R K Holmes
Journal: Microbiol Rev Date: 1987-06

3. The mode of action of Shiga toxin on peptide elongation of eukaryotic protein synthesis.

Authors: T G Obrig; T P Moran; J E Brown
Journal: Biochem J Date: 1987-06-01 Impact factor: 3.857

4. Morphologic evaluation of the effects of Shiga toxin and E coli Shiga-like toxin on the rabbit intestine.

Authors: K P Keenan; D D Sharpnack; H Collins; S B Formal; A D O'Brien
Journal: Am J Pathol Date: 1986-10 Impact factor: 4.307

5. Ionic requirements for entry of Shiga toxin from Shigella dysenteriae 1 into cells.

Authors: K Sandvig; J E Brown
Journal: Infect Immun Date: 1987-02 Impact factor: 3.441

6. Isolation and characterization of monoclonal antibodies to Shiga toxin.

Authors: D E Griffin; M K Gentry; J E Brown
Journal: Infect Immun Date: 1983-07 Impact factor: 3.441

7. Hybridization of Escherichia coli producing Shiga-like toxin I, Shiga-like toxin II, and a variant of Shiga-like toxin II with synthetic oligonucleotide probes.

Authors: J E Brown; O Sethabutr; M P Jackson; S Lolekha; P Echeverria
Journal: Infect Immun Date: 1989-09 Impact factor: 3.441