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Literature DB >> 7047395

Expression of Treponema pallidum antigens in Escherichia coli K-12.

Abstract

A colony bank of recombinant plasmids harboring Treponema pallidum DNA inserts has been established in Escherichia coli K-12. By using an in situ immunoassay, we identified four E. coli clones that expressed T. pallidum antigens. Thus, recombinant DNA technology may provide powerful new tools for studying the pathogenesis of T. pallidum infection.

Entities: Disease Species

Mesh：

Substances：

Year: 1982 PMID： 7047395 PMCID： PMC551462 DOI： 10.1128/iai.36.3.1238-1241.1982

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

16 in total

1. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival.

Authors: T J Fitzgerald; J N Miller; J A Sykes
Journal: Infect Immun Date: 1975-05 Impact factor: 3.441

2. Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.

Authors: L W Enquist; M J Madden; P Schiop-Stanley; G F Vande Woude
Journal: Science Date: 1979-02-09 Impact factor: 47.728

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

4. Homology of the gene coding for outer membrane lipoprotein within various Gram-negative bacteria.

Authors: K Nakamura; R M Pirtle; M Inouye
Journal: J Bacteriol Date: 1979-01 Impact factor: 3.490

5. Parasitism by virulent Treponema pallidum of host cell surfaces.

Authors: N S Hayes; K E Muse; A M Collier; J B Baseman
Journal: Infect Immun Date: 1977-07 Impact factor: 3.441

6. Capacity of virulent Treponema pallidum (Nichols) for deoxyribonucleic acid synthesis.

Authors: J B Baseman; J C Nichols; S Mogerley
Journal: Infect Immun Date: 1979-02 Impact factor: 3.441

7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

8. Genetics of Treponema: relationship between Treponema pallidum and five cultivable treponemes.

Authors: R Miao; A H Fieldsteel
Journal: J Bacteriol Date: 1978-01 Impact factor: 3.490

9. Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems.

Authors: A H Fieldsteel; F A Becker; J G Stout
Journal: Infect Immun Date: 1977-10 Impact factor: 3.441

10. Isolation of Escherichia coli mutants defective in enzymes of membrane lipid synthesis.

Authors: C R Raetz
Journal: Proc Natl Acad Sci U S A Date: 1975-06 Impact factor: 11.205

24 in total

1. Molecular cloning and characterization of DNA from human intestinal spirochetes.

Authors: G Dettori; R Burioni; R Grillo; P Cattani
Journal: Eur J Epidemiol Date: 1992-03 Impact factor: 8.082

Review 2. The Treponema pallidum Outer Membrane.

Authors: Justin D Radolf; Sanjiv Kumar
Journal: Curr Top Microbiol Immunol Date: 2018 Impact factor: 4.291

3. Purification and characterization of a cloned protease-resistant Treponema pallidum-specific antigen.

Authors: T E Fehniger; A M Walfield; T M Cunningham; J D Radolf; J N Miller; M A Lovett
Journal: Infect Immun Date: 1984-11 Impact factor: 3.441

4. Molecular cloning and characterization of a 35.5-kilodalton lipoprotein of Treponema pallidum.

Authors: C L Hubbard; F C Gherardini; P J Bassford; L V Stamm
Journal: Infect Immun Date: 1991-04 Impact factor: 3.441

5. Overproduction and purification of Treponema pallidum recombinant-DNA-derived proteins TmpA and TmpB and their potential use in serodiagnosis of syphilis.

Authors: L M Schouls; O E Ijsselmuiden; J Weel; J D van Embden
Journal: Infect Immun Date: 1989-09 Impact factor: 3.441

6. Cloning and expression of Legionella pneumophila antigens in Escherichia coli.

Authors: N C Engleberg; D J Drutz; B I Eisenstein
Journal: Infect Immun Date: 1984-05 Impact factor: 3.441

7. Serological characterization and gene localization of an Escherichia coli-expressed 37-kilodalton Treponema pallidum antigen.

Authors: G C Rodgers; W J Laird; S R Coates; D H Mack; M Huston; J J Sninsky
Journal: Infect Immun Date: 1986-07 Impact factor: 3.441

8. Identification and purification of a recombinant Treponema pallidum basic membrane protein antigen expressed in Escherichia coli.

Authors: W S Dallas; P H Ray; J Leong; C D Benedict; L V Stamm; P J Bassford
Journal: Infect Immun Date: 1987-05 Impact factor: 3.441

9. Role of disulfide bonds in the oligomeric structure and protease resistance of recombinant and native Treponema pallidum surface antigen 4D.

Authors: J D Radolf; L A Borenstein; J Y Kim; T E Fehniger; M A Lovett
Journal: J Bacteriol Date: 1987-04 Impact factor: 3.490

10. Enzyme linked immunosorbent assays with Treponema pallidum or axial filament of T phagedenis biotype Reiter as antigen: evaluation as screening tests for syphilis.

Authors: R V van Eijk; H E Menke; G J Tideman; E Stolz
Journal: Genitourin Med Date: 1986-12