Literature DB >> 7045809

Laser crosslinking of E. coli RNA polymerase and T7 DNA.

C A Harrison, D H Turner, D C Hinkle.   

Abstract

The first photochemical crosslinking of a protein to a nucleic acid using laser excitation is reported. A single, 120 mJ, 20 ns pulse at 248 nm crosslinks about 10% of bound E. coli RNA polymerase to T7 DNA under the conditions studied. The crosslinking yield depends on mercaptoethanol concentration, and is a linear function of laser intensity. The protein subunits crosslinked to DNA are beta, beta' and sigma.

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Year:  1982        PMID： 7045809      PMCID： PMC320618          DOI： 10.1093/nar/10.7.2399

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

Review 1.  Statistical time events in enzymes: a physical assessment.

Authors:  G Careri; P Fasella; E Gratton
Journal:  CRC Crit Rev Biochem       Date:  1975-08

2.  Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.

Authors:  D C Hinkle; M J Chamberlin
Journal:  J Mol Biol       Date:  1972-09-28       Impact factor: 5.469

3.  Ultraviolet light-induced stable complexes of DNA and DNA polymerase.

Authors:  A Markovitz
Journal:  Biochim Biophys Acta       Date:  1972-11-09

4.  Induction of stable linkage between the deoxyribonucleic acid dependent ribonucleic acid polymerase and d(A-T)n-d(A-T)n by ultraviolet light.

Authors:  G F Strniste; D A Smith
Journal:  Biochemistry       Date:  1974-01-29       Impact factor: 3.162

5.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

6.  Determination of molecular weight distributions of DNA by means of sedimentation in a sucrose gradient.

Authors:  G P van der Schans; J B Aten
Journal:  Anal Biochem       Date:  1969-10-15       Impact factor: 3.365

7.  The mechanism of photochemical addition of cysteine to uracil and formation of dihydrouracil.

Authors:  T Jellinek; R B Johns
Journal:  Photochem Photobiol       Date:  1970-05       Impact factor: 3.421

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

Authors:  R R Burgess; J J Jendrisak
Journal:  Biochemistry       Date:  1975-10-21       Impact factor: 3.162

10.  A mixed photoproduct of thymine and cysteine: 5-S-cysteine, 6-hydrothymine.

Authors:  K C Smith
Journal:  Biochem Biophys Res Commun       Date:  1970       Impact factor: 3.575
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  14 in total

1.  A specific and efficient photoreaction between E. coli RNA polymerase and T+1 in the lacUV5 or deoP1 promoter.

Authors:  C Jeppesen; K F Jensen; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

2.  Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation.

Authors:  D Angelov; S I Dimitrov; V R Russanova; E Keskinova; I G Pashev
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

3.  Conformational changes in E. coli RNA polymerase during promoter recognition.

Authors:  K L Brodolin; V M Studitsky; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1993-12-11       Impact factor: 16.971

4.  Identification of proteins interacting with newly replicated DNA in SV40-infected cells by UV-induced DNA-protein cross-linking.

Authors:  K Tsutsui; S Watanabe; M Katagiri; T Oda
Journal:  Nucleic Acids Res       Date:  1983-07-25       Impact factor: 16.971

5.  Protein-DNA cross-linking at the lac promoter.

Authors:  M Buckle; J Geiselmann; A Kolb; H Buc
Journal:  Nucleic Acids Res       Date:  1991-02-25       Impact factor: 16.971

6.  In vivo genomic footprinting analysis reveals that the complex Bradyrhizobium japonicum fixRnifA promoter region is differently occupied by two distinct RNA polymerase holoenzymes.

Authors:  H Barrios; R Grande; L Olvera; E Morett
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-03       Impact factor: 11.205

7.  Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.

Authors:  D S Gilmour; J T Lis
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  The region of phage T4 genes 34, 33 and 59: primary structures and organization on the genome.

Authors:  S Hahn; U Kruse; W Rüger
Journal:  Nucleic Acids Res       Date:  1986-12-09       Impact factor: 16.971

9.  Efficiency and pattern of UV pulse laser-induced RNA-RNA cross-linking in the ribosome.

Authors:  Tatjana Shapkina; Simon Lappi; Stefan Franzen; Paul Wollenzien
Journal:  Nucleic Acids Res       Date:  2004-03-03       Impact factor: 16.971

10.  Ultraviolet laser-induced cross-linking in peptides.

Authors:  Gabriella Leo; Carlo Altucci; Sandrine Bourgoin-Voillard; Alfredo M Gravagnuolo; Rosario Esposito; Gennaro Marino; Catherine E Costello; Raffaele Velotta; Leila Birolo
Journal:  Rapid Commun Mass Spectrom       Date:  2013-07-30       Impact factor: 2.419
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