Literature DB >> 7045592

Involvement of the nusB gene products in transcription of Escherichia coli tryptophan operon in vitro.

K Kuroki, S Ishii, Y Kano, T Miyashita, K Nishi, F Imamoto.   

Abstract

Synthesis of trp mRNA in vitro directed by plasmid DNA carrying the entire trp operon was studied using crude protein extracts (S-100) from Escherichia coli strains carrying the nusA or nusB mutation or both. It was found that the levels of trp mRNA transcribed from the promoter-distal genes (trpCBA) relative to that from the promoter-proximal genes (trpED) was markedly lower with extracts from the nus- mutants than that from the nus+ strain. Kinetic experiments suggest that termination of RNA transcripts at intragenic transcriptional barriers is prevented by the nus gene products from allowing efficient expression of the operon.

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Year:  1982        PMID： 7045592     DOI： 10.1007/bf00330816

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  19 in total

1.  Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.

Authors:  H Kung; C Spears; H Weissbach
Journal:  J Biol Chem       Date:  1975-02-25       Impact factor: 5.157

2.  DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation.

Authors:  T Zarucki-Schulz; C Jerez; G Goldberg; H F Kung; K H Huang; N Brot; H Weissbach
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

Review 3.  A comprehensive molecular map of bacteriophage lambda.

Authors:  E H Szybalski; W Szybalski
Journal:  Gene       Date:  1979-11       Impact factor: 3.688

4.  Origin of replication of colicin E1 plasmid DNA.

Authors:  J I Tomizawa; H Ohmori; R E Bird
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

5.  Transcription of the tryptophan operon in polarity mutants of Escherichia coli. I. Characterization of the tryptophan messenger RNA of polar mutants.

Authors:  F Imamoto; C Yanofsky
Journal:  J Mol Biol       Date:  1967-08-28       Impact factor: 5.469

6.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

7.  Characterization of lambdatrp-transducing bacteriophages made in vitro.

Authors:  A S Hopkins; N E Murray; W J Brammar
Journal:  J Mol Biol       Date:  1976-11-15       Impact factor: 5.469

8.  Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function.

Authors:  C P Georgopoulos; J Swindle; F Keppel; M Ballivet; R Bisig; H Eisen
Journal:  Mol Gen Genet       Date:  1980

9.  L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.

Authors:  J Greenblatt; J Li; S Adhya; D I Friedman; L S Baron; B Redfield; H F Kung; H Weissbach
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

10.  In vivo correlation between DNA supercoiling and transcription.

Authors:  Y Kano; T Miyashita; H Nakamura; K Kuroki; A Nagata; F Imamoto
Journal:  Gene       Date:  1981-03       Impact factor: 3.688
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  9 in total

1.  Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation.

Authors:  T Taura; C Ueguchi; K Shiba; K Ito
Journal:  Mol Gen Genet       Date:  1992-09

2.  Multivalent regulation of the nusA operon of Escherichia coli.

Authors:  A Ishihama; A Honda; H Nagasawa-Fujimori; R E Glass; T Maekawa; F Imamoto
Journal:  Mol Gen Genet       Date:  1987-02

3.  Purification of the NusB gene product of Escherichia coli K12.

Authors:  T Maekawa; T Nagase; F Imamoto; S Ishii
Journal:  Mol Gen Genet       Date:  1985

4.  Molecular cloning and nucleotide sequencing of the nusB gene of E. coli.

Authors:  S Ishii; E Hatada; T Maekawa; F Imamoto
Journal:  Nucleic Acids Res       Date:  1984-06-25       Impact factor: 16.971

5.  Isolation and characterization of nucleoid proteins from Escherichia coli.

Authors:  K Yamazaki; A Nagata; Y Kano; F Imamoto
Journal:  Mol Gen Genet       Date:  1984

6.  tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.

Authors:  S Ishii; K Kuroki; F Imamoto
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

7.  The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.

Authors:  S Ishii; M Ihara; T Maekawa; Y Nakamura; H Uchida; F Imamoto
Journal:  Nucleic Acids Res       Date:  1984-04-11       Impact factor: 16.971

8.  Direct expression of hepatitis B surface antigen gene in E. coli.

Authors:  Y Fujisawa; Y Ito; R Sasada; Y Ono; K Igarashi; R Marumoto; M Kikuchi; Y Sugino
Journal:  Nucleic Acids Res       Date:  1983-06-11       Impact factor: 16.971

9.  Identification of Rumen Microbial Genes Involved in Pathways Linked to Appetite, Growth, and Feed Conversion Efficiency in Cattle.

Authors:  Joana Lima; Marc D Auffret; Robert D Stewart; Richard J Dewhurst; Carol-Anne Duthie; Timothy J Snelling; Alan W Walker; Tom C Freeman; Mick Watson; Rainer Roehe
Journal:  Front Genet       Date:  2019-08-08       Impact factor: 4.599
  9 in total

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