Literature DB >> 6330694

Molecular cloning and nucleotide sequencing of the nusB gene of E. coli.

S Ishii, E Hatada, T Maekawa, F Imamoto.   

Abstract

The nusB gene of E. coli has been cloned in plasmid pBR322. Using genetic complementation as an assay for the gene, its location in subclones was analyzed, and the nucleotide sequence of this gene and its flanking regions was determined. The coding region consists of 417 base pairs (bp), which specify a protein of 139 amino acids, and the calculated molecular weight of the nusB protein is 15,702. The nusB protein contains 20 acidic and 21 basic amino acids. A significant promoter sequence was not found to be located in the region upstream from the translational initiation codon. The possibility that the nusB gene consists of an operon is discussed. After the coding region, there is a G-C rich inverted repeat sequence followed by a run of Ts, which could be a transcriptional terminator of the nusB gene.

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Year:  1984        PMID： 6330694      PMCID： PMC318893          DOI： 10.1093/nar/12.12.4987

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Nucleotide sequence of an RNA polymerase binding site at an early T7 promoter.

Authors:  D Pribnow
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

2.  Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.

Authors:  H Kung; C Spears; H Weissbach
Journal:  J Biol Chem       Date:  1975-02-25       Impact factor: 5.157

3.  Termination factor for RNA synthesis.

Authors:  J W Roberts
Journal:  Nature       Date:  1969-12-20       Impact factor: 49.962

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Authors:  R R Burgess; J J Jendrisak
Journal:  Biochemistry       Date:  1975-10-21       Impact factor: 3.162

5.  Cooperative effects of bacterial mutations affecting lambda N gene expression. I. Isolation and characterization of a nusB mutant.

Authors:  D I Friedman; M Baumann; L S Baron
Journal:  Virology       Date:  1976-08       Impact factor: 3.616

6.  Construction of a recombinant bacterial plasmid containing pro-alpha 1(I) collagen DNA sequences.

Authors:  T Yamamoto; M E Sobel; S L Adams; V E Avvedimento; R DiLauro; I Pastan; B de Crombrugghe; A Showalter; D Pesciotta; P Fietzek; B Olsen
Journal:  J Biol Chem       Date:  1980-03-25       Impact factor: 5.157

7.  The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.

Authors:  R A Johnson; T F Walseth
Journal:  Adv Cyclic Nucleotide Res       Date:  1979

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors:  J Shine; L Dalgarno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

10.  Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd.

Authors:  H Schaller; C Gray; K Herrmann
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205
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  10 in total

1.  Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation.

Authors:  T Taura; C Ueguchi; K Shiba; K Ito
Journal:  Mol Gen Genet       Date:  1992-09

Review 2.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 3.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

4.  Purification of the NusB gene product of Escherichia coli K12.

Authors:  T Maekawa; T Nagase; F Imamoto; S Ishii
Journal:  Mol Gen Genet       Date:  1985

5.  Structural and functional analyses of the transcription-translation proteins NusB and NusE.

Authors:  D L Court; T A Patterson; T Baker; N Costantino; X Mao; D I Friedman
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

6.  ssaD1, a suppressor of secA51(Ts) that renders growth of Escherichia coli cold sensitive, is an early amber mutation in the transcription factor gene nusB.

Authors:  T Rajapandi; D Oliver
Journal:  J Bacteriol       Date:  1994-07       Impact factor: 3.490

Review 7.  Interactions of bacteriophage and host macromolecules in the growth of bacteriophage lambda.

Authors:  D I Friedman; E R Olson; C Georgopoulos; K Tilly; I Herskowitz; F Banuett
Journal:  Microbiol Rev       Date:  1984-12

8.  Evidence that ribosomal protein S10 itself is a cellular component necessary for transcription antitermination by phage lambda N protein.

Authors:  A Das; B Ghosh; S Barik; K Wolska
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

9.  Identification and purification of the N gene product of bacteriophage phi 80.

Authors:  K Kanemoto; S Tanaka; T Miyashita; A Matsushiro
Journal:  Mol Gen Genet       Date:  1986-12

10.  Measurements of translation initiation from all 64 codons in E. coli.

Authors:  Ariel Hecht; Jeff Glasgow; Paul R Jaschke; Lukmaan A Bawazer; Matthew S Munson; Jennifer R Cochran; Drew Endy; Marc Salit
Journal:  Nucleic Acids Res       Date:  2017-04-20       Impact factor: 16.971
  10 in total

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