Literature DB >> 7041126

Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis.

S Stahl, P MacKay, M Magazin, S A Bruce, K Murray.   

Abstract

Fragments of hepatitis B virus DNA cloned in plasmid pBR322 carrying the gene for the viral core antigen have been placed under the control of the lac promoter of Escherichia coli. Several of the new recombinants direct higher levels of synthesis of the antigen, but the degree of enhancement varies with the different structures of the plasmids and hence the mRNAs produced. The antigen in crude bacterial lysates is a satisfactory diagnostic reagent for antibodies to the core antigen in serum samples.

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Year:  1982        PMID： 7041126      PMCID： PMC346024          DOI： 10.1073/pnas.79.5.1606

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  Mucopeptide biosynthesis by minicells of Escherichia coli.

Authors:  J N Reeve
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

3.  Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors:  E M Lederberg; S N Cohen
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

4.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

5.  Carbohydrates in hepatitis B antigen.

Authors:  C J Burrell; E Proudfoot; G A Keen; B P Marmion
Journal:  Nat New Biol       Date:  1973-06-27

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.

Authors:  C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1965-07       Impact factor: 11.205

8.  Microtiter solid-phase radioimmunoassay for hepatitis B antigen.

Authors:  R H Purcell; D C Wong; H J Alter; P V Holland
Journal:  Appl Microbiol       Date:  1973-10

9.  Application of a screening test for antibody the hepatitis B core antigen.

Authors:  B J Cohen; Y E Cossart
Journal:  J Clin Pathol       Date:  1977-08       Impact factor: 3.411

10.  Synthesis of cell envelope components by anucleate cells (minicells) of Bacillus subtilis.

Authors:  G Mertens; J N Reeve
Journal:  J Bacteriol       Date:  1977-03       Impact factor: 3.490
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  39 in total

1.  Contamination of a recombinant hepatitis B virus nucleocapsid preparation with a human B-cell activator.

Authors:  Peter Vanlandschoot; Freya Van Houtte; Benedikte Serruys; Geert Leroux-Roels
Journal:  J Virol       Date:  2006-12-06       Impact factor: 5.103

2.  Non-canonical binding of an antibody resembling a naïve B cell receptor immunoglobulin to hepatitis B virus capsids.

Authors:  Norman R Watts; Giovanni Cardone; Joe G Vethanayagam; Naiqian Cheng; Catharina Hultgren; Stephen J Stahl; Alasdair C Steven; Matti Sällberg; Paul T Wingfield
Journal:  J Mol Biol       Date:  2008-04-09       Impact factor: 5.469

3.  The affinities of monoclonal antibodies against core antigen of hepatitis B virus.

Authors:  U Apsalons; V Bichko
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

4.  Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: implications for morphogenesis and organization of encapsidated RNA.

Authors:  A Zlotnick; N Cheng; S J Stahl; J F Conway; A C Steven; P T Wingfield
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

5.  Characterization of the major duck hepatitis B virus core particle protein.

Authors:  J Pugh; A Zweidler; J Summers
Journal:  J Virol       Date:  1989-03       Impact factor: 5.103

6.  Hepatitis B synthetic immunogen comprised of nucleocapsid T-cell sites and an envelope B-cell epitope.

Authors:  D R Milich; J L Hughes; A McLachlan; G B Thornton; A Moriarty
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

7.  Hepatitis B core antigen synthesised in Escherichia coli: its use for antibody screening in patients attending a clinic for sexually transmitted diseases.

Authors:  B J Cohen; P A Litton; P P Mortimer; P Simmons
Journal:  J Hyg (Lond)       Date:  1984-10

8.  Expression of hepatitis B virus core antigen gene in Saccharomyces cerevisiae: synthesis of two polypeptides translated from different initiation codons.

Authors:  A Miyanohara; T Imamura; M Araki; K Sugawara; N Ohtomo; K Matsubara
Journal:  J Virol       Date:  1986-07       Impact factor: 5.103

9.  Immunogenicity of recombinant core particles of hepatitis B virus containing epitopes of human immunodeficiency virus 1 core antigen.

Authors:  R Ulrich; G P Borisova; E Gren; I Berzin; P Pumpen; R Eckert; V Ose; H Siakkou; E J Gren; R von Baehr
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

10.  The duck hepatitis B virus pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation.

Authors:  H J Schlicht; J Salfeld; H Schaller
Journal:  J Virol       Date:  1987-12       Impact factor: 5.103
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