Literature DB >> 7039149

How to make a hybridoma.

E A Lerner.   

Abstract

The use of monoclonal antibodies produced by the hybridoma technique has had a profound impact on the biological and medical sciences. While the general technique for the production of monoclonal antibodies is available from the numerous papers which report their use, there is a need for a source which describes the procedure and its pitfalls in detail so that an investigator who decides to make hybridomas can do so quickly and easily. This guide provides such a source.

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Year:  1981        PMID： 7039149      PMCID： PMC2596021     

Source DB:  PubMed          Journal:  Yale J Biol Med        ISSN： 0044-0086


  10 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

2.  SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors:  J W LITTLEFIELD
Journal:  Science       Date:  1964-08-14       Impact factor: 47.728

3.  Monoclonal xenogeneic antibodies to mouse leukocyte antigens: identification of macrophage-specific and other differentiation antigens.

Authors:  T Springer; G Galfre; D Secher; C Milstein
Journal:  Curr Top Microbiol Immunol       Date:  1978       Impact factor: 4.291

4.  A better cell line for making hybridomas secreting specific antibodies.

Authors:  M Shulman; C D Wilde; G Köhler
Journal:  Nature       Date:  1978-11-16       Impact factor: 49.962

5.  The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors.

Authors:  W E Wright
Journal:  Exp Cell Res       Date:  1978-03-15       Impact factor: 3.905

Review 6.  Antibody production by hybridomas.

Authors:  J W Goding
Journal:  J Immunol Methods       Date:  1980       Impact factor: 2.303

7.  Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.

Authors:  G Galfrè; C Milstein; B Wright
Journal:  Nature       Date:  1979-01-11       Impact factor: 49.962

8.  Continuous production of monoclonal rheumatoid factor by EBV-transformed lymphocytes.

Authors:  M Steinitz; G Izak; S Cohen; M Ehrenfeld; I Flechner
Journal:  Nature       Date:  1980-10-02       Impact factor: 49.962

9.  Production of human hybridomas secreting antibodies to measles virus.

Authors:  C M Croce; A Linnenbach; W Hall; Z Steplewski; H Koprowski
Journal:  Nature       Date:  1980-12-04       Impact factor: 49.962

10.  Monoclonal antibody against an Ir gene product?

Authors:  E A Lerner; L A Matis; C A Janeway; P P Jones; R H Schwartz; D B Murphy
Journal:  J Exp Med       Date:  1980-10-01       Impact factor: 14.307
  10 in total
  5 in total

1.  Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide.

Authors:  T F Eckert; T R Kozel
Journal:  Infect Immun       Date:  1987-08       Impact factor: 3.441

2.  Interaction of endothelial cell growth factor with heparin: characterization by receptor and antibody recognition.

Authors:  A B Schreiber; J Kenney; W J Kowalski; R Friesel; T Mehlman; T Maciag
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

3.  Detection of high levels of heparin binding growth factor-1 (acidic fibroblast growth factor) in inflammatory arthritic joints.

Authors:  H Sano; R Forough; J A Maier; J P Case; A Jackson; K Engleka; T Maciag; R L Wilder
Journal:  J Cell Biol       Date:  1990-04       Impact factor: 10.539

Review 4.  Pharmacokinetics of Monoclonal Antibodies.

Authors:  Josiah T Ryman; Bernd Meibohm
Journal:  CPT Pharmacometrics Syst Pharmacol       Date:  2017-07-29

5.  Acquisition of antigens characteristic of adult pericentral hepatocytes by differentiating fetal hepatoblasts in vitro.

Authors:  A L Bennett; K E Paulson; R E Miller; J E Darnell
Journal:  J Cell Biol       Date:  1987-09       Impact factor: 10.539
  5 in total

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