Literature DB >> 7031077

Value of passive immune hemolysis for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli.

T Tsukamoto, Y Kinoshita, S Taga, Y Takeda, T Miwatani.   

Abstract

The method of passive immune hemolysis of Evans and Evans (Infect. Immun. 16:604-609, 1977) for detection of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli was modified. A total of 373 strains of E. coli were tested by this method using materials obtained by treating the cells with polymyxin B and rabbit antiserum against cholera enterotoxin, purified by affinity gel column coupled with purified cholera enterotoxin, in N-hydroxyethylpiperazine-N'-2-ethanesulfonic acid buffer (pH 6.7). The results correlated very well with those obtained in an assay with Chinese hamster ovary cells. It is concluded that passive immune hemolysis is useful as a routine clinical method for identifying E. coli strains that produce heat-labile enterotoxin.

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Year:  1980        PMID： 7031077      PMCID： PMC273694          DOI： 10.1128/jcm.12.6.768-771.1980

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Factors influencing heat-labile Escherichia coli enterotoxin activity.

Authors:  D H Mundell; C R Anselmo; R M Wishnow
Journal:  Infect Immun       Date:  1976-08       Impact factor: 3.441

2.  Response of the rabbit ileal loop to cell-free products from Escherichia coli enteropathogenic for swine.

Authors:  H W Moon; S C Whipp; G W Engstrom; A L Baetz
Journal:  J Infect Dis       Date:  1970-02       Impact factor: 5.226

3.  Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus.

Authors:  T Honda; M Shimizu; Y Takeda; T Miwatani
Journal:  Infect Immun       Date:  1976-10       Impact factor: 3.441

4.  Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

Authors:  D A Sack; R B Sack
Journal:  Infect Immun       Date:  1975-02       Impact factor: 3.441

5.  Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man.

Authors:  D J Evans; D G Evans; S L Gorbach
Journal:  Infect Immun       Date:  1973-11       Impact factor: 3.441

6.  Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis.

Authors:  D J Evans; D G Evans
Journal:  Infect Immun       Date:  1977-05       Impact factor: 3.441

7.  Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.

Authors:  H B Greenberg; D A Sack; W Rodriguez; R B Sack; R G Wyatt; A R Kalica; R L Horswood; R M Chanock; A Z Kapikian
Journal:  Infect Immun       Date:  1977-09       Impact factor: 3.441

8.  Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.

Authors:  M B Serafim; A F Pestana de Castro; M H Lemos dos Reis; L R Trabulsi
Journal:  Infect Immun       Date:  1979-06       Impact factor: 3.441

9.  Isolation and characterization of homogeneous heat-labile enterotoxins with high specific activity from Escherichia coli cultures.

Authors:  J D Clements; R A Finkelstein
Journal:  Infect Immun       Date:  1979-06       Impact factor: 3.441

10.  Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors:  R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal:  Infect Immun       Date:  1974-08       Impact factor: 3.441
  10 in total
  11 in total

1.  Identity of hemolysins produced by Vibrio cholerae non-O1 and V. cholerae O1, biotype El Tor.

Authors:  K Yamamoto; Y Ichinose; N Nakasone; M Tanabe; M Nagahama; J Sakurai; M Iwanaga
Journal:  Infect Immun       Date:  1986-03       Impact factor: 3.441

2.  Automatic and manual latex agglutination tests for measurement of cholera toxin and heat-labile enterotoxin of Escherichia coli.

Authors:  T Ito; S Kuwahara; T Yokota
Journal:  J Clin Microbiol       Date:  1983-01       Impact factor: 5.948

3.  Purification and some properties of a non-o1 Vibrio cholerae enterotoxin that is identical to cholera enterotoxin.

Authors:  K Yamamoto; Y Takeda; T Miwatani; J P Craig
Journal:  Infect Immun       Date:  1983-03       Impact factor: 3.441

4.  Modified Elek test for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli.

Authors:  T Honda; S Taga; Y Takeda; T Miwatani
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

5.  Detection by a staphylococcal coagglutination test of heat-labile enterotoxin-producing enterotoxigenic Escherichia coli.

Authors:  T Honda; R Samakoses; C Sornchai; Y Takeda; T Miwatani
Journal:  J Clin Microbiol       Date:  1983-04       Impact factor: 5.948

6.  Improved GM1-enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

Authors:  P A Ristaino; M M Levine; C R Young
Journal:  J Clin Microbiol       Date:  1983-10       Impact factor: 5.948

7.  Rapid test for identification of heat-labile enterotoxin-producing Escherichia coli colonies.

Authors:  R A Finkelstein; Z Yang
Journal:  J Clin Microbiol       Date:  1983-07       Impact factor: 5.948

8.  In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.

Authors:  Y Takeda; T Honda; S Taga; T Miwatani
Journal:  Infect Immun       Date:  1981-11       Impact factor: 3.441

9.  Detection of heat-labile (LT) enterotoxin of enterotoxigenic Escherichia coli by the radial immune hemolysis test: a modification for clinical use.

Authors:  T Yano; M S Oliveira; C F Fontes; A C de Almeida; A F de Castro
Journal:  Med Microbiol Immunol       Date:  1982       Impact factor: 3.402

10.  Storage of Escherichia coli thermolabile enterotoxin on filter papers for assay by immune haemolysis reactions.

Authors:  L C Ricci; C F Fontes; P S Siqueira; A F Pestana de Castro
Journal:  Med Microbiol Immunol       Date:  1984       Impact factor: 3.402
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