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Literature DB >> 7022454

Direct selection procedure for the isolation of functional centromeric DNA.

Abstract

Several plasmids, each containing a segment of DNA capable of conferring mitotic stability on the ars1 vector YRp7 replicating in yeast (Saccharomyces cerevisiae), have been isolated from a yeast genomic library by direct selection for stable plasmid maintenance. The mitotic-stabilizing sequences fall into two classes: (i) segments of the yeast "2-micrometers" plasmid and (ii) DNA segments containing yeast centromeric DNA. Plasmids of the first class segregate 4+:0- through meiosis whereas the centromere plasmids segregate predominantly 2+:2-, as typical chromosomes. Previously identified centromeres from chromosomes II (CEN3) and XI (CEN11), along with the CEN11-linked gene MET14, can be isolated by this direct selection procedure. In addition, several other unidentified centromere DNAs were obtained. There are no detectable sequence homologies between CEN3 and CEN11 DNAs nor do these DNAs hybridize to the other yeast centromere regions.

Entities: Gene Species

Mesh：

Substances：
DNA

Year: 1981 PMID： 7022454 PMCID： PMC319652 DOI： 10.1073/pnas.78.6.3760

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Small-scale techniques for the analysis of recombinant plasmids.

Authors: A G Hepburn; J Hindley
Journal: J Biochem Biophys Methods Date: 1979-10

3. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors: J R Broach; J N Strathern; J B Hicks
Journal: Gene Date: 1979-12 Impact factor: 3.688

4. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors: L Clarke; J Carbon
Journal: Cell Date: 1976-09 Impact factor: 41.582

5. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

Authors: K A Nasmyth; S I Reed
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

6. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.

Authors: A J Kingsman; L Clarke; R K Mortimer; J Carbon
Journal: Gene Date: 1979-10 Impact factor: 3.688

7. Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.

Authors: J N Strathern; C S Newlon; I Herskowitz; J B Hicks
Journal: Cell Date: 1979-10 Impact factor: 41.582

4. Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions.

Authors: B E Diehl; J R Pringle
Journal: Genetics Date: 1991-02 Impact factor: 4.562

10. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.

Authors: R J Bram; R D Kornberg
Journal: Mol Cell Biol Date: 1987-01 Impact factor: 4.272

Direct selection procedure for the isolation of functional centromeric DNA.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Small-scale techniques for the analysis of recombinant plasmids.

3. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

4. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

5. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

6. Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.

7. Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.

8. High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.

9. Isolation of a yeast centromere and construction of functional small circular chromosomes.

10. Isolation of the centromere-linked CDC10 gene by complementation in yeast.

1. Replication forks pause at yeast centromeres.

2. Purification of a protein binding to the CDEI subregion of Saccharomyces cerevisiae centromere DNA.

3. Characterization of a centromere-linked recombination hot spot in Saccharomyces cerevisiae.

4. Molecular analysis of Saccharomyces cerevisiae chromosome I: identification of additional transcribed regions and demonstration that some encode essential functions.

5. The study of a rDNA replicator in Saccharomyces.

Review 6. Yeast chromosome replication and segregation.

7. A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.

8. A mutant of Saccharomyces cerevisiae with impaired maintenance of centromeric plasmids.

9. Structure and sequence of the centromeric DNA of chromosome 4 in Saccharomyces cerevisiae.

10. Isolation of a Saccharomyces cerevisiae centromere DNA-binding protein, its human homolog, and its possible role as a transcription factor.