Literature DB >> 7022127

DNA sequence of a plasmid-encoded dihydrofolate reductase.

G Swift, B J McCarthy, F Heffron.   

Abstract

The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.

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Year:  1981        PMID： 7022127     DOI： 10.1007/bf00428733

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  23 in total

1.  Two distinct types of trimethoprim-resistant dihydrofolate reductase specified by R-plasmids of different compatibility groups.

Authors:  K H Pattishall; J Acar; J J Burchall; F W Goldstein; R J Harvey
Journal:  J Biol Chem       Date:  1977-04-10       Impact factor: 5.157

2.  The purification and properties of the trimethoprim-resistant dihydrofolate reductase mediated by the R-factor, R388.

Authors:  S G Amyes; J T Smith
Journal:  Eur J Biochem       Date:  1976-01-15

3.  The amino acid sequence of the trimethoprim-resistant dihydrofolate reductase specified in Escherichia coli by R-plasmid R67.

Authors:  D Stone; S L Smith
Journal:  J Biol Chem       Date:  1979-11-10       Impact factor: 5.157

4.  Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.

Authors:  J W Zolg; U J Hänggi; H G Zachau
Journal:  Mol Gen Genet       Date:  1978-08-04

5.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

6.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors:  D B Clewell; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1969-04       Impact factor: 11.205

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Protein expression in Escherichia coli minicells containing recombinant plasmids specifying trimethoprim-resistant dihydrofolate reductases.

Authors:  M E Fling; L P Elwell
Journal:  J Bacteriol       Date:  1980-02       Impact factor: 3.490

9.  Trimethoprim resistance determined by R factors.

Authors:  M P Fleming; N Datta; R N Grüneberg
Journal:  Br Med J       Date:  1972-03-18

10.  Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.

Authors:  F Bolivar; R L Rodriguez; M C Betlach; H W Boyer
Journal:  Gene       Date:  1977       Impact factor: 3.688
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  38 in total

1.  New gene cassettes for trimethoprim resistance, dfr13, and Streptomycin-spectinomycin resistance, aadA4, inserted on a class 1 integron.

Authors:  P V Adrian; C J Thomson; K P Klugman; S G Amyes
Journal:  Antimicrob Agents Chemother       Date:  2000-02       Impact factor: 5.191

2.  Erythromycin esterase gene ere(A) is located in a functional gene cassette in an unusual class 2 integron.

Authors:  Latefa Biskri; Didier Mazel
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191

3.  Development of a DNA probe from the deoxyribonucleotide sequence of a 3-N-aminoglycoside acetyltransferase [AAC(3)-I] resistance gene.

Authors:  F C Tenover; K L Phillips; T Gilbert; P Lockhart; P J O'Hara; J J Plorde
Journal:  Antimicrob Agents Chemother       Date:  1989-04       Impact factor: 5.191

4.  Mapping of trimethoprim resistance genes from epidemiologically related plasmids.

Authors:  I G Campbell; B J Mee
Journal:  Antimicrob Agents Chemother       Date:  1987-09       Impact factor: 5.191

5.  Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.

Authors:  Carlos Revilla; M Pilar Garcillán-Barcia; Raúl Fernández-López; Nicholas R Thomson; Mandy Sanders; Martin Cheung; Christopher M Thomas; Fernando de la Cruz
Journal:  Antimicrob Agents Chemother       Date:  2008-02-11       Impact factor: 5.191

6.  Plasmids bearing hfq and the hns-like gene stpA complement hns mutants in modulating arginine decarboxylase gene expression in Escherichia coli.

Authors:  X Shi; G N Bennett
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

7.  The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants.

Authors:  I T Paulsen; T G Littlejohn; P Rådström; L Sundström; O Sköld; G Swedberg; R A Skurray
Journal:  Antimicrob Agents Chemother       Date:  1993-04       Impact factor: 5.191

8.  Myxococcus xanthus sasN encodes a regulator that prevents developmental gene expression during growth.

Authors:  D Xu; C Yang; H B Kaplan
Journal:  J Bacteriol       Date:  1998-12       Impact factor: 3.490

9.  Expression of plasmid R388-encoded type II dihydrofolate reductase as a dominant selective marker in Saccharomyces cerevisiae.

Authors:  A Miyajima; I Miyajima; K Arai; N Arai
Journal:  Mol Cell Biol       Date:  1984-03       Impact factor: 4.272

10.  Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements.

Authors:  P Rådström; O Sköld; G Swedberg; J Flensburg; P H Roy; L Sundström
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490
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