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Literature DB >> 7021600

Cultural method for large-scale screening for Chlamydia trachomatis genital infection.

Abstract

Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be examined concurrently. It is sufficiently sensitive to form the basis of a chlamydial culture service for patients attending Sexually Transmitted Diseases (STD) Clinics.

Entities: Chemical Disease Species

Mesh：

Year: 1981 PMID： 7021600 PMCID： PMC493799 DOI： 10.1136/jcp.34.7.712

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

11 in total

1. Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents).

Authors: F W Johnson; D Hobson
Journal: J Hyg (Lond) Date: 1976-06

Review 2. The rôle of Chlamydia trachomatis in genital-tract and associated diseases.

Authors: D Taylor-Robinson; B J Thomas
Journal: J Clin Pathol Date: 1980-03 Impact factor: 3.411

3. Micro cell culture method for isolation of Chlamydia trachomatis.

Authors: D E McComb; C I Puzniak
Journal: Appl Microbiol Date: 1974-10

4. A comparison of staining techniques for demonstrating group A chlamydia in tissue culture.

Authors: F W Johnson
Journal: Med Lab Technol Date: 1975-07

5. Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract.

Authors: P Reeve; J Owen; J D Oriel
Journal: J Clin Pathol Date: 1975-11 Impact factor: 3.411

6. The need for a chlamydial culture service.

Authors: J R Willcox; P G Fisk; J Barrow; D Barlow
Journal: Br J Vener Dis Date: 1979-08

7. Techniques for culturing and determining antimicrobial susceptibility of Chlamydia trachomatis.

Authors: T R Rota
Journal: Arch Androl Date: 1980-02

8. Prospective study of maternal and infantile infection with Chlamydia trachomatis.

Authors: M R Hammerschlag; M Anderka; D Z Semine; D McComb; W M McCormack
Journal: Pediatrics Date: 1979-08 Impact factor: 7.124

9. The isolation of Chlamydia subgroup A (Chlamydia trachomatis) in irradiated McCoy cells.

Authors: S J Richmond
Journal: Med Lab Technol Date: 1974-01

10. Value and feasibility of screening women attending STD clinics for cervical chlamydial infections.

Authors: S J Richmond; I D Paul; P K Taylor
Journal: Br J Vener Dis Date: 1980-04

11 in total

1. Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.

Authors: T Krech; M Bleckmann; R Paatz
Journal: J Clin Microbiol Date: 1989-10 Impact factor: 5.948

8. Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.

Authors: W E Stamm; M Tam; M Koester; L Cles
Journal: J Clin Microbiol Date: 1983-04 Impact factor: 5.948

9. Quantitative study of Chlamydia trachomatis in genital infection.

Authors: H Mallinson; O P Arya; A D Goddard
Journal: Br J Vener Dis Date: 1982-02

10. Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.

Authors: N R Mohammed; I B Hillary
Journal: J Clin Pathol Date: 1984-06 Impact factor: 3.411