Literature DB >> 7021370

A simple procedure to obtain yeast hexokinase free of glucosephosphate isomerase and mannosephosphate isomerase.

V Cabrera, C Pascual, L S Herrera.   

Abstract

A hexokinase preparation was obtained from a Saccharomyces cerevisiae mutant strain deficient in glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) by precipitation with ammonium sulfate. The supernatant fraction corresponding to 40-60 % saturation showed the lowest content in GPI and MPI activity. The fraction was used without further purification in the determination of glucose, either free or in a mixture with fructose and mannose. The results were similar to those obtained with pure commercial hexokinase.

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Year:  1981        PMID： 7021370     DOI： 10.1007/bf02927364

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  4 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Genetic and biochemical studies of phosphomannose isomerase deficient mutants of Saccharomyces cerevisiae.

Authors:  L S Herrera; C Pascual; X Alvarez
Journal:  Mol Gen Genet       Date:  1976-03-22

3.  Yeast hexokinase: substrate-induced association--dissociation reactions in the binding of glucose to hexokinase P-II.

Authors:  J G Hoggett; G L Kellett
Journal:  Eur J Biochem       Date:  1976-06-15

4.  Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase.

Authors:  S Kang; A Markovitz
Journal:  J Bacteriol       Date:  1967-02       Impact factor: 3.490
  4 in total

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