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Literature DB >> 7017346

An improved method for two-dimensional gel-electrophoresis: analysis of mutationally altered ribosomal proteins of Escherichia coli.

Abstract

An improved method for the two-dimensional electrophoretic analysis of ribosomal proteins on acrylamide gel slabs has been developed by combining the procedures for the first dimension of Mets and Bogorad (1974) and for the second dimension of Kaltschmidt and Wittmann (1970) and by introducing several modifications. Ribosomal proteins of various Escherichia coli mutants have been analyzed by the new method. Advantages are that (1) it requires only small amounts of protein (100-200 micrograms 70S ribosomal proteins), (2) reproducibility is very high, and (3) it makes it easier to identify mutational alterations in proteins S10, L4, L10, and L21 which hardly migrate out of the sample gel with our previous electrophoresis procedure. Furthermore, the new method can be nicely adapted to analysis of the ribosomal proteins from other organisms, such as Bacilli or yeast.

Entities: Chemical Species

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Year: 1981 PMID： 7017346 DOI： 10.1007/bf00425603

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

15 in total

1. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli.

Authors: S Isono; K Isono
Journal: Mol Gen Genet Date: 1978-09-20

2. Mutational alterations in 50 proteins of the Escherichia coli ribosome.

Authors: E R Dabbs
Journal: Mol Gen Genet Date: 1978-09-20

3. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.

Authors: M Kitakawa; E R Dabbs; K Isono
Journal: J Bacteriol Date: 1979-06 Impact factor: 3.490

4. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence.

Authors: R Hasenbank; C Guthrie; G Stöffler; H G Wittmann; L Rosen; D Apirion
Journal: Mol Gen Genet Date: 1973-12-14

5. Altered S5 and S20 ribosomal proteins in revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.

Authors: H G Wittmann; G Stöffler
Journal: Mol Gen Genet Date: 1974

6. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.

Authors: L J Mets; L Bogorad
Journal: Anal Biochem Date: 1974-01 Impact factor: 3.365

7. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.

Authors: S J Hardy; C G Kurland; P Voynow; G Mora
Journal: Biochemistry Date: 1969-07 Impact factor: 3.162

8. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.

Authors: K Isono; J Krauss; Y Hirota
Journal: Mol Gen Genet Date: 1976-12-22

9. 30S subunit mutations relieving restriction of ribosomal misreading caused by L6 mutations.

Authors: H Hummel; W Piepersberg; A Böck
Journal: Mol Gen Genet Date: 1980

10. Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae.

Authors: W Stöcklein; W Piepersberg
Journal: Curr Genet Date: 1980-04 Impact factor: 3.886

45 in total

Review 1. Protein kinase associated with ribosomes of streptomycetes.

Authors: K Mikulík; E Zhoulanova; Q K Hoang; J Janecek; S Bezousková
Journal: Folia Microbiol (Praha) Date: 1999 Impact factor: 2.099

2. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer.

Authors: G Diedrich; C M Spahn; U Stelzl; M A Schäfer; T Wooten; D E Bochkariov; B S Cooperman; R R Traut; K H Nierhaus
Journal: EMBO J Date: 2000-10-02 Impact factor: 11.598

7. Nonbridging phosphate oxygens in 16S rRNA important for 30S subunit assembly and association with the 50S ribosomal subunit.

Authors: Srikanta Ghosh; Simpson Joseph
Journal: RNA Date: 2005-04-05 Impact factor: 4.942

An improved method for two-dimensional gel-electrophoresis: analysis of mutationally altered ribosomal proteins of Escherichia coli.

1. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli.

2. Mutational alterations in 50 proteins of the Escherichia coli ribosome.

3. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.

4. Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence.

5. Altered S5 and S20 ribosomal proteins in revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.

6. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.

7. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.

8. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins.

9. 30S subunit mutations relieving restriction of ribosomal misreading caused by L6 mutations.

10. Altered ribosomal protein L29 in a cycloheximide-resistant strain of Saccharomyces cerevisiae.

Review 1. Protein kinase associated with ribosomes of streptomycetes.

2. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer.

3. DnaK-facilitated ribosome assembly in Escherichia coli revisited.

4. Isolation and characterization of Bacillus stearothermophilus 30S and 50S ribosomal protein mutations.

5. The involvement of base 1054 in 16S rRNA for UGA stop codon dependent translational termination.

6. Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein.

7. Nonbridging phosphate oxygens in 16S rRNA important for 30S subunit assembly and association with the 50S ribosomal subunit.

8. Role of ribosome degradation in the death of starved Escherichia coli cells.

9. The sarcin-ricin loop of 23S rRNA is essential for assembly of the functional core of the 50S ribosomal subunit.

10. Overexpression of RbfA in the absence of the KsgA checkpoint results in impaired translation initiation.