Literature DB >> 7016879

Purification and primary structure of a polypeptide with multiplication-stimulating activity from rat liver cell cultures. Homology with human insulin-like growth factor II.

H Marquardt, G J Todaro, L E Henderson, S Oroszlan.   

Abstract

A low molecular weight polypeptide with multiplication-stimulating activity (MSA) was isolated from serum-free media conditioned by a clone of normal Buffalo rat liver cells. The purification of MSA was achieved by gel permeation chromatography of the acid-soluble growth-promoting activity on Bio-Gel P-10 in 1 M acetic acid and followed by reversed phase high pressure liquid chromatography on muBondapak C18 support using a linear gradient of acetonitrile in 0.05% trifluoroacetic acid. The primary structure of MSA has been determined. MSA is a single chain polypeptide of 67 residues, with a calculated molecular weight of 7,484, and displays 93% homology with the functionally related human insulin-like growth factor II (IGF-II). A comparison between the sequences of rat MSA and human IGF-II reveals only five amino acid substitutions. Based on the extensive amino acid sequence homology, we propose the term rat IGF-II for this newly isolated polypeptide.

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Year:  1981        PMID： 7016879

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  38 in total

Review 1.  Molecular biology of the insulin-like growth factors. Relevance to nervous system function.

Authors:  J E Hepler; P K Lund
Journal:  Mol Neurobiol       Date:  1990 Spring-Summer       Impact factor: 5.590

Review 2.  Somatomedins: chemical and functional characteristics of the different molecular forms.

Authors:  A Barreca; F Minuto
Journal:  J Endocrinol Invest       Date:  1989-04       Impact factor: 4.256

3.  Delineation of atypical insulin receptors from classical insulin and type I insulin-like growth factor receptors in human placenta.

Authors:  H A Jonas; A J Cox; L C Harrison
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

4.  A few distinct 'molecular sandwiches' are basis for structural and functional similarities of subspecies of interferon alpha and of families of growth-promoting hormones.

Authors:  A D Inglot; W Popik; E Piasecki; J Czyrski
Journal:  Mol Biol Rep       Date:  1986       Impact factor: 2.316

5.  Interactions of insulin, insulinlike growth factor II, and platelet-derived growth factor in erythropoietic culture.

Authors:  N Dainiak; S Kreczko
Journal:  J Clin Invest       Date:  1985-09       Impact factor: 14.808

6.  Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum.

Authors:  G L Francis; L C Read; F J Ballard; C J Bagley; F M Upton; P M Gravestock; J C Wallace
Journal:  Biochem J       Date:  1986-01-01       Impact factor: 3.857

7.  Receptors for multiplication-stimulating activity (MSA) on rabbit chondrocytes.

Authors:  I K Ashton; J H Soul
Journal:  Calcif Tissue Int       Date:  1984-09       Impact factor: 4.333

8.  The role of the insulin receptor in mediating the insulin-stimulated growth response in Reuber H-35 cells.

Authors:  J W Koontz
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

9.  Insulin and growth factor effects on c-fos expression in normal and protein kinase C-deficient 3T3-L1 fibroblasts and adipocytes.

Authors:  D J Stumpo; P J Blackshear
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

10.  Structure of the insulin-like growth factor receptor in chicken embryo fibroblasts.

Authors:  M Kasuga; E Van Obberghen; S P Nissley; M M Rechler
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205
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