Literature DB >> 7012155

A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli.

M M Cox, K McEntee, I R Lehman.   

Abstract

A simple and rapid three-step procedure for the large scale purification of the recA protein of Escherichia coli is described. The method depends primarily on a single chromatographic step which is highly specific for recA protein: elution by ATP from single-stranded DNA cellulose. With this procedure, gram quantities of recA protein, greater than 99% pure, can be reproducibility prepared for biochemical and biophysical analysis.

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Year:  1981        PMID： 7012155

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  52 in total

1.  Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis.

Authors:  A Maor-Shoshani; N B Reuven; G Tomer; Z Livneh
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  Purification and properties of the RuvA and RuvB proteins of Escherichia coli.

Authors:  I R Tsaneva; G Illing; R G Lloyd; S C West
Journal:  Mol Gen Genet       Date:  1992-10

3.  A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments.

Authors:  M Dutreix; B Burnett; A Bailone; C M Radding; R Devoret
Journal:  Mol Gen Genet       Date:  1992-04

4.  Immunological quantification of RecA protein in cell extracts of E. coli after exposure to chemical mutagens or UV radiation.

Authors:  I Fridrichová; A Kovarík; O Rosskopfová
Journal:  Folia Microbiol (Praha)       Date:  1992       Impact factor: 2.099

5.  Interaction of recA protein with left-handed Z-DNA.

Authors:  P Krishna; A R Morgan; J H van de Sande
Journal:  Biochem J       Date:  1991-05-01       Impact factor: 3.857

6.  A complementary pair of rapid molecular screening assays for RecA activities.

Authors:  Andrew M Lee; Tim J Wigle; Scott F Singleton
Journal:  Anal Biochem       Date:  2007-04-20       Impact factor: 3.365

7.  10 nm RecA protein filaments formed in the presence of Mg2+ and ATP gamma S may contain RNA.

Authors:  J C Register; J Griffith
Journal:  Mol Gen Genet       Date:  1985

8.  Molar absorptivity and A1 cm (1%) values for proteins at selected wavelengths of the ultraviolet and visible regions. XXII.

Authors:  D M Kirschenbaum
Journal:  Appl Biochem Biotechnol       Date:  1982-11       Impact factor: 2.926

9.  Homologous pairing of single-stranded circular DNAs catalyzed by recA protein.

Authors:  S L Keener; K McEntee
Journal:  Nucleic Acids Res       Date:  1984-08-10       Impact factor: 16.971

10.  P. mirabilis RecA protein catalyses cleavage of E. coli LexA protein and the lambda repressor in vitro.

Authors:  S C West; J W Little
Journal:  Mol Gen Genet       Date:  1984
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