Literature DB >> 7009755

An inexpensive, portable, battery-operated photometer for the reading of ELISA tests in microtitration plates.

G A Rook, C H Cameron.   

Abstract

We described an inexpensive, portable, battery-operated photometer which can read the enzyme-linked immunosorbent assay in 96-well microtitre trays. An example is given of its use to measure IgG antibody to human collagen type II in a serum sample from a rheumatoid arthritis patient known to have a high titre of antibody to cartilage by immunofluorescence. When the same samples were read first with the portable photometer and then in a spectrophotometer (Unicam SP 1800), the accuracy of the two devices was comparable, but the readings were obtained far more quickly in the photometer.

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Year:  1981        PMID: 7009755     DOI: 10.1016/0022-1759(81)90086-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  6 in total

1.  Lymphocytes from patients with rheumatoid arthritis produce agalactosylated IgG in vitro.

Authors:  K B Bodman; N Sumar; L E Mackenzie; D A Isenberg; F C Hay; I M Roitt; P M Lydyard
Journal:  Clin Exp Immunol       Date:  1992-06       Impact factor: 4.330

2.  A monoclonal antibody raised by immunising mice with group A streptococci binds to agalactosyl IgG from rheumatoid arthritis.

Authors:  G A Rook; J Steele; T Rademacher
Journal:  Ann Rheum Dis       Date:  1988-03       Impact factor: 19.103

3.  A transient rise in agalactosyl IgG correlating with free interleukin 2 receptors, during episodes of erythema nodosum leprosum.

Authors:  E Filley; A Andreoli; J Steele; M Waters; D Wagner; D Nelson; K Tung; T Rademacher; R Dwek; G A Rook
Journal:  Clin Exp Immunol       Date:  1989-06       Impact factor: 4.330

4.  Probe colorimeter for quantitating enzyme-linked immunosorbent assays and other colorimetric assays performed with microplates.

Authors:  S B Ackerman; E A Kelley
Journal:  J Clin Microbiol       Date:  1983-03       Impact factor: 5.948

5.  Agalactosyl IgG in inflammatory bowel disease: correlation with C-reactive protein.

Authors:  R Dubé; G A Rook; J Steele; R Brealey; R Dwek; T Rademacher; J Lennard-Jones
Journal:  Gut       Date:  1990-04       Impact factor: 23.059

6.  T-cell-dependent polyclonal activation by soluble mycobacterial extracts of B cells in peripheral blood mononuclear cell populations from leprosy patients and normal donors.

Authors:  B Fleming; G A Rook
Journal:  Immunology       Date:  1982-12       Impact factor: 7.397

  6 in total

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