Literature DB >> 7008986

A radiometric assay for the combined measurement of phagocytosis and intracellular killing of Candida albicans.

C G Bridges, G L Dasilva, M Yamamura, H Valdimarsson.   

Abstract

A radiometric assay for combined measurement of phagocytosis and intracellular killing of Candida albicans by human polymorphonuclear leucocytes (PMNs) is presented. The assay, based upon the incorporation of 3H-uridine into the micro-organisms, makes it possible to measure phagocytosis and intracellular killing simultaneously but independently in a single sample. Thus it is possible to determine in a single assay whether increased survival of the micro-organism is due to reduced ingestion or reduced ability of the PMN to kill. The assay is objective, quantitative and convenient for clinical application. It is also suitable for analysing the effects of various agents, including serum factors and drugs, on PMN function.

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Year:  1980        PMID： 7008986      PMCID： PMC1537093     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  11 in total

1.  Quantitation of maximal bactericidal capability in human neutrophils.

Authors:  C C Clawson; J E Repine
Journal:  J Lab Clin Med       Date:  1976-08

2.  Phagocytosis measured as inhibition of uridine uptake: a method that distinguishes between surface adherence and ingestion.

Authors:  C Lam; G E Mathison
Journal:  J Med Microbiol       Date:  1979-11       Impact factor: 2.472

3.  Phagocytosis measured as inhibition of uridine uptake by Candida albicans.

Authors:  M Yamamura; J Boler; H Valdimarsson
Journal:  J Immunol Methods       Date:  1977       Impact factor: 2.303

4.  Interaction of Candida albicans with human leukocytes and serum.

Authors:  R I Lehrer; M J Cline
Journal:  J Bacteriol       Date:  1969-06       Impact factor: 3.490

5.  A chromium release assay for phagocytic killing of Candida albicans.

Authors:  M Yamamura; J Boler; H Valdimarsson
Journal:  J Immunol Methods       Date:  1976       Impact factor: 2.303

6.  A micromethod for measuring neutrophil candidacidal activity in neonates.

Authors:  R Oseas; R I Lehrer
Journal:  Pediatr Res       Date:  1978-08       Impact factor: 3.756

7.  Kinetics of phagocytosis and intracellular killing of Candida albicans by human granulocytes and monocytes.

Authors:  P C Leijh; M T van den Barselaar; R van Furth
Journal:  Infect Immun       Date:  1977-08       Impact factor: 3.441

8.  Participation of C3 in intracellular killing of Candida albicans.

Authors:  M Yamamura; H Valdimarsson
Journal:  Scand J Immunol       Date:  1977       Impact factor: 3.487

9.  Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein beta1H for cleavage of C3b and C4b in solution.

Authors:  M K Pangburn; R D Schreiber; H J Müller-Eberhard
Journal:  J Exp Med       Date:  1977-07-01       Impact factor: 14.307

10.  Modulation of the alternative complement pathways by beta 1 H globulin.

Authors:  K Whaley; S Ruddy
Journal:  J Exp Med       Date:  1976-11-02       Impact factor: 14.307
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  18 in total

1.  Effect of feverfew on phagocytosis and killing of Candida guilliermondii by neutrophils.

Authors:  L M Williamson; D M Harvey; K J Sheppard; J Fletcher
Journal:  Inflammation       Date:  1988-02       Impact factor: 4.092

2.  Neutrophil phagocytosis and killing in insulin-dependent diabetes.

Authors:  R M Wilson; W G Reeves
Journal:  Clin Exp Immunol       Date:  1986-02       Impact factor: 4.330

3.  New microbial and host factors in disseminated gonococcal infection: case report.

Authors:  G E Forster; A J Pinching; C A Ison; C S Easmon; P E Munday
Journal:  Genitourin Med       Date:  1987-06

4.  Neutrophil function in chronic liver disease.

Authors:  A C Campbell; M W Dronfield; P J Toghill; W G Reeves
Journal:  Clin Exp Immunol       Date:  1981-07       Impact factor: 4.330

5.  In vitro and in vivo impact of a new glycosphingolipid on neutrophils.

Authors:  E Tubaro; C Croce; G Cavallo; L Belogi; G Guida; C Santiangeli; M G Cifone; A Santoni; F Mainiero
Journal:  Agents Actions       Date:  1994-10

6.  Candida albicans stimulates arachidonic acid liberation from alveolar macrophages through alpha-mannan and beta-glucan cell wall components.

Authors:  M Castro; N V Ralston; T I Morgenthaler; M S Rohrbach; A H Limper
Journal:  Infect Immun       Date:  1994-08       Impact factor: 3.441

7.  Effects of broad-spectrum antibiotics on colonization of gastrointestinal tracts of mice by Candida albicans.

Authors:  G Samonis; H Anastassiadou; M Dassiou; Y Tselentis; G P Bodey
Journal:  Antimicrob Agents Chemother       Date:  1994-03       Impact factor: 5.191

8.  Bacteraemia due to recurrent reinfection with Staphylococcus epidermidis associated with defective opsonisation and procidin function in serum.

Authors:  L R Baker; A L Brown; J R Stephenson; S Tabaqchali; M Zatouroff; J M Parkin; A J Pinching
Journal:  J Clin Pathol       Date:  1993-05       Impact factor: 3.411

9.  Combined effect of fluconazole and thymosin alpha 1 on systemic candidiasis in mice immunosuppressed by morphine treatments.

Authors:  P di Francesco; R Gaziano; I A Casalinuovo; L Belogi; A T Palamara; C Favalli; E Garaci
Journal:  Clin Exp Immunol       Date:  1994-09       Impact factor: 4.330

10.  Roles of antibody and complement in the bactericidal activity of mouse peritoneal exudate neutrophils.

Authors:  P H Hart; L K Spencer; N L Hill; P J McDonald; J J Finlay-Jones
Journal:  Br J Exp Pathol       Date:  1987-02
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