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Literature DB >> 7003308

Amber-mutants of plasmid mini-F defective in replication.

Abstract

Amber mutants of the mini-F plasmid pML31 were isolated with the mutagen hydroxylamine. Under non-permissive conditions amber mutants segregate and show no incorporation of label into supercoiled plasmid DNA in double-label experiments. Wild-type and one mutant of mini-F were integrated by recombinant DNA techniques into the single EcoRI site of plasmid vector pBR322. Plasmid specific proteins were labeled in minicells and analysed by SDS-PAGE. A 34,000 dalton molecular weight protein was identified to be missing in the amber mutant of plasmid mini-F.

Entities: Chemical

Mesh：

Substances：

Year: 1980 PMID： 7003308 DOI： 10.1007/bf00267370

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

23 in total

1. MINIATURE escherichia coli CELLS DEFICIENT IN DNA.

Authors: H I Adler; W D Fisher; A Cohen; A A Hardigree
Journal: Proc Natl Acad Sci U S A Date: 1967-02 Impact factor: 11.205

2. F plasmid incompatibility and copy number genes: their map locations and interactions.

Authors: J J Manis; B C Kline
Journal: Plasmid Date: 1978-09 Impact factor: 3.466

3. Temperature-dependent and amber copy mutants of plasmid R1drd-19 in Escherichia coli.

Authors: P Gustafsson; K Nordström
Journal: Plasmid Date: 1978-02 Impact factor: 3.466

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

5. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].

Authors: F Cuzin; F Jacob
Journal: Ann Inst Pasteur (Paris) Date: 1967-04

6. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

Authors: D I Smith; F R Blattner; J Davies
Journal: Nucleic Acids Res Date: 1976-02 Impact factor: 16.971

7. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids.

Authors: G O Humphreys; G A Willshaw; H R Smith; E S Anderson
Journal: Mol Gen Genet Date: 1976-04-23

8. The transposon Tn1 as a probe for studying ColE1 structure and function.

Authors: G Dougan; D Sherratt
Journal: Mol Gen Genet Date: 1977-03-07

9. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.

Authors: I Andrés; P M Slocombe; F Cabello; J K Timmis; R Lurz; H J Burkardt; K N Timmis
Journal: Mol Gen Genet Date: 1979-01-05

Review 4. Regulation of plasmid replication.

Authors: J R Scott
Journal: Microbiol Rev Date: 1984-03

Amber-mutants of plasmid mini-F defective in replication.

1. MINIATURE escherichia coli CELLS DEFICIENT IN DNA.

2. F plasmid incompatibility and copy number genes: their map locations and interactions.

3. Temperature-dependent and amber copy mutants of plasmid R1drd-19 in Escherichia coli.

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

5. [Mutations of the F episome of Escherichia coli K 12. II. Mutants with thermosensitive replication].

6. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

7. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids.

8. The transposon Tn1 as a probe for studying ColE1 structure and function.

9. Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.

10. Altered DNA-protein relaxation complex in a replication mutant of plasmid ColE1.

1. The repeated sequences (incB) preceding the protein E gene of plasmid mini-F are essential for replication.

2. The miniF plasmid C protein: sequence, purification and DNA binding.

3. Transfer inhibition of RP4 by F factor.

Review 4. Regulation of plasmid replication.

5. Complementation of replication-deficient deletion derivatives of plasmid mini-F.

6. An essential gene for replication of the mini-F plasmid from origin I.

7. An amber replication mutant of F plasmid mapped in the minimal replication region.

8. Genetic evidence that control of F replication is negative.

9. Identification of the pifC gene and its role in negative control of F factor pif gene expression.

10. Novobiocin-induced elimination of F'lac and mini-F plasmids from Escherichia coli.