| Literature DB >> 6384731 |
Abstract
We constructed a series of defective mini-F plasmids, which have deletion(s) in the replication origin I and/or origin II, and their derivatives, which do not produce F3 protein, by insertion of the XhoI fragment of Tn5 into the XhoI site at 41.0 F (kilobases on the coordinate map of F-plasmid). Using these mutant mini-F plasmids, we found that F3 protein is essential for the replication of mini-F from origin I, but not from origin II.Mesh:
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Year: 1984 PMID: 6384731 DOI: 10.1007/bf00334092
Source DB: PubMed Journal: Mol Gen Genet ISSN: 0026-8925