Literature DB >> 7000680

The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM.

M Pinder, A J Musoke, W I Morrison, G E Roelants.   

Abstract

Mouse spleen cells from animals immunized with bovine peripheral blood lymphocytes were fused to X63 . Ag8 myeloma cells and the activity of one of the resulting myeloma hybrids was characterized. The product of this clone (B5/4.1.4) binds to pentameric bovine IgM isolated from serum but not to serum IgG1 or IgG2. This reagent also binds to cell surface (monomeric) IgM and can be used in immunofluorescence assays to enumerate IgM-bearing cells in lymphoid cell suspensions and to examine B lymphocytes or B lymphocyte derived cells in tissue sections.

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Year:  1980        PMID： 7000680      PMCID： PMC1458052     

Source DB:  PubMed          Journal:  Immunology        ISSN： 0019-2805            Impact factor:   7.397


  11 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

2.  Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes.

Authors:  A F Williams; G Galfrè; C Milstein
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

3.  Active synthesis of immunoglobulin receptors for antigen by T lymphocytes.

Authors:  G E Roelants; A Rydén; L B Hägg; F Loor
Journal:  Nature       Date:  1974-01-11       Impact factor: 49.962

4.  Immunochemical quantitation of antigens by single radial immunodiffusion.

Authors:  G Mancini; A O Carbonara; J F Heremans
Journal:  Immunochemistry       Date:  1965-09

5.  Immunofluorescence studies of a possible prethymic T-cell differentiation in congenitally athymic (nude) mice.

Authors:  F Loor; G E Roelants
Journal:  Ann N Y Acad Sci       Date:  1975-06-30       Impact factor: 5.691

6.  Crossreacting determinants in variant-specific surface antigens of African trypanosomes.

Authors:  A F Barbet; T C McGuire
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

7.  Stabilization and preservation of the antigenic specificity of Trypanosoma (Trypanozoon) brucei variant specific surface antigens by mild fixation techniques.

Authors:  V M Nantulya; J J Doyle
Journal:  Acta Trop       Date:  1977-12       Impact factor: 3.112

8.  Protein A reactivity of various mammalian immunoglobulins.

Authors:  J Goudswaard; J A van der Donk; A Noordzij; R H van Dam; J P Vaerman
Journal:  Scand J Immunol       Date:  1978       Impact factor: 3.487

9.  The bovine lymphoid system: binding and stimulation of peripheral blood lymphocytes by lectins.

Authors:  T W Pearson; G E Roelants; L B Lundin; K S Mayor-Withey
Journal:  J Immunol Methods       Date:  1979       Impact factor: 2.303

10.  A myeloma hybrid producing antibody specific for an allotypic determinant on "IgD-like" molecules of the mouse.

Authors:  T Pearson; G Galfrè; A Ziegler; C Milstein
Journal:  Eur J Immunol       Date:  1977-10       Impact factor: 5.532
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  11 in total

1.  Theileria annulata and T. parva infect and transform different bovine mononuclear cells.

Authors:  R L Spooner; E A Innes; E J Glass; C G Brown
Journal:  Immunology       Date:  1989-02       Impact factor: 7.397

2.  The properties and functional activity of non-lymphoid cells from bovine afferent (peripheral) lymph.

Authors:  D L Emery; N D MacHugh; J A Ellis
Journal:  Immunology       Date:  1987-10       Impact factor: 7.397

3.  Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens.

Authors:  C L Baldwin; N D Machugh; J A Ellis; J Naessens; J Newson; W I Morrison
Journal:  Immunology       Date:  1988-03       Impact factor: 7.397

4.  Proliferation and lymphocyte stimulatory capacity of Theileria-infected lymphoblastoid cells before and after the elimination of intracellular parasites.

Authors:  M Pinder; S Kar; K S Withey; L B Lundin; G E Roelants
Journal:  Immunology       Date:  1981-09       Impact factor: 7.397

5.  In vitro secretion of bovine immunoglobulins during pokeweed mitogen or pokeweed mitogen and antigen activation of lymphocytes.

Authors:  J O Olobo; S J Black
Journal:  Vet Res Commun       Date:  1989       Impact factor: 2.459

6.  High and low responsiveness of bovine lymphocytes to Trypanosoma brucei in vitro: lack of correlation with resistance to trypanosomiasis.

Authors:  F Fumoux; T Traore-Leroux; R Queval; M Pinder; G E Roelants
Journal:  Immunology       Date:  1985-01       Impact factor: 7.397

7.  The bovine autologous Theileria mixed leucocyte reaction: influence of monocytes and phenotype of the parasitized stimulator cell on proliferation and parasite specificity.

Authors:  B M Goddeeris; W I Morrison
Journal:  Immunology       Date:  1987-01       Impact factor: 7.397

8.  Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.

Authors:  J Naessens; J Newson; D J Williams; V Lutje
Journal:  Immunology       Date:  1988-04       Impact factor: 7.397

9.  Expression of Tac antigen component of bovine interleukin-2 receptor in different leukocyte populations infected with Theileria parva or Theileria annulata.

Authors:  D A Dobbelaere; T D Prospero; I J Roditi; C Kelke; I Baumann; M Eichhorn; R O Williams; J S Ahmed; C L Baldwin; H Clevers
Journal:  Infect Immun       Date:  1990-12       Impact factor: 3.441

10.  Bovine immune response to Theileria parva: neutralizing antibodies to sporozoites.

Authors:  A J Musoke; V M Nantulya; G Buscher; R A Masake; B Otim
Journal:  Immunology       Date:  1982-04       Impact factor: 7.397
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